Pain is a subjective and complex physiological response of the body to tissue damage. Leaves of Myristica fragrans Houtt contain active compounds such as flavonoids, alkaloids, tannins, and myristicin, which may affect analgesic properties. The aim of study aims to evaluate the analgesic effect and effective dose of leaf ethanol extract Myristica fragrans Houtt using the hot-plate method. A total of thirty mice were randomly divided into 6 groups, namely negative control (Na-CMC), positive control (paracetamol), and four treatment groups with extract doses of 200, 300, 400, and 500 mg/kgBW. Pain responses were observed through jumping and paw licking behavior at 30-minute intervals over 120 minutes, with cut-off 5 minutes. Data were analyzed statistically using Kruskal-Wallis with a follow-up Mann-Whitney test. The results showed that all doses of ethanol extract Myristica fragrans Houtt were significantly different (p0.005). The analgesic effectiveness of ethanol extract at a dose of 500 mg/kg BW was the greatest at 55.36%. The research results concluded that ethanol extract of Myristica fragrans Houtt had an analgesic effect and the most effective dose is 500 mg/kg BW
