A Krogh

A Marchler-Bauer

A Mcleod

AJ Drummond

AJ Haverkort

AJ Phillips

B Kloareg

BJ Haas

BM Tyler

C Katsaros

CA Lévesque

Claire M. M. Gachon

CMM Gachon

D Takemoto

Dee A. Carter

DG Müller

E Gaulin

E White

EPC Rocha

F Maumus

F Weinberger

FC Küpper

FK Sparrow

Frithjof C. Küpper

G Gremme

G Guerriero

G Michel

H Li

HJG Meijer

I Badreddine

J Perez-Vilar

JA West

JM Cock

KC Chou

KD Lafferty

L Baxter

Laura Grenville-Briggs

Lieven Sterck

LJ Grenville-Briggs

M Frada

M Mort-Bontemps

M Strittmatter

MA Larkin

Martina Strittmatter

O Berteau

O Emanuelsson

P Rice

P van West

Pieter van West

PV Peña

R Oliva

S Baldauf

S Bartnicki-Garcia

S Bhattacharjee

S Grouffaud

S Hunter

S Kamoun

S Sekimoto

SC Whisson

SD Taverna

SF Altschul

T Tonon

V Bulone

V Roeder

X Huang

XJ Min

PLoS ONE

English

PubMed

Brown algae are the predominant primary producers in coastal habitats, and like land plants are subject to disease and parasitism. Eurychasma dicksonii is an abundant, and probably cosmopolitan, obligate biotrophic oomycete pathogen of marine brown algae. Oomycetes (or water moulds) are pathogenic or saprophytic non-photosynthetic Stramenopiles, mostly known for causing devastating agricultural and aquacultural diseases. Whilst molecular knowledge is restricted to crop pathogens, pathogenic oomycetes actually infect hosts from most eukaryotic lineages. Molecular evidence indicates that Eu. dicksonii belongs to the most early-branching oomycete clade known so far. Therefore Eu. dicksonii is of considerable interest due to its presumed environmental impact and phylogenetic position. Here we report the first large scale functional molecular data acquired on the most basal oomycete to date. 9873 unigenes, totalling over 3.5Mb of sequence data, were produced from Sanger-sequenced and pyrosequenced EST libraries of infected Ectocarpus siliculosus. 6787 unigenes (70%) were of algal origin, and 3086 (30%) oomycete origin. 57% of Eu. dicksonii sequences had no similarity to published sequence data, indicating that this dataset is largely unique. We were unable to positively identify sequences belonging to the RXLR and CRN groups of oomycete effectors identified in higher oomycetes, however we uncovered other unique pathogenicity factors. These included putative algal cell wall degrading enzymes, cell surface proteins, and cyclophilin-like proteins. A first look at the host response to infection has also revealed movement of the host nucleus to the site of infection as well as expression of genes responsible for strengthening the cell wall, and secretion of proteins such as protease inhibitors. We also found evidence of transcriptional reprogramming of E. siliculosus transposable elements and of a viral gene inserted in the host genome

Grenville-Briggs, Laura

Gachon, Claire MM

Strittmatter, Martina

Sterck, Lieven

Küpper, Frithjof C

van West, Pieter

Ghent University Academic Bibliography

A molecular insight into algal-oomycete warfare: cDNA analysis of Ectocarpus siliculosus infected with the basal oomycete Eurychasma dicksonii

Brown algae are the predominant primary producers in coastal habitats, and like land plants are subject to disease and parasitism. Eurychasma dicksonii is an abundant, and probably cosmopolitan, obligate biotrophic oomycete pathogen of marine brown algae. Oomycetes (or water moulds) are pathogenic or saprophytic non-photosynthetic Stramenopiles, mostly known for causing devastating agricultural and aquacultural diseases. Whilst molecular knowledge is restricted to crop pathogens, pathogenic oomycetes actually infect hosts from most eukaryotic lineages. Molecular evidence indicates that Eu. dicksonii belongs to the most early-branching oomycete clade known so far. Therefore Eu. dicksonii is of considerable interest due to its presumed environmental impact and phylogenetic position. Here we report the first large scale functional molecular data acquired on the most basal oomycete to date. 9873 unigenes, totalling over 3.5 Mb of sequence data, were produced from Sanger-sequenced and pyrosequenced EST libraries of infected Ectocarpus siliculosus. 6787 unigenes (70%) were of algal origin, and 3086 (30%) oomycete origin. 57% of Eu. dicksonii sequences had no similarity to published sequence data, indicating that this dataset is largely unique. We were unable to positively identify sequences belonging to the RXLR and CRN groups of oomycete effectors identified in higher oomycetes, however we uncovered other unique pathogenicity factors. These included putative algal cell wall degrading enzymes, cell surface proteins, and cyclophilin-like proteins. A first look at the host response to infection has also revealed movement of the host nucleus to the site of infection as well as expression of genes responsible for strengthening the cell wall, and secretion of proteins such as protease inhibitors. We also found evidence of transcriptional reprogramming of E. siliculosus transposable elements and of a viral gene inserted in the host genome

Claire M M Gachon

Frithjof C Küpper

Directory of Open Access Journals

A molecular insight into algal-oomycete warfare: cDNA analysis of Ectocarpus siliculosus infected with the basal oomycete Eurychasma dicksonii.

Brown algae are the predominant primary producers in coastal habitats, and like land plants are subject to disease and parasitism. Eurychasma dicksonii is an abundant, and probably cosmopolitan, obligate biotrophic oomycete pathogen of marine brown algae. Oomycetes (or water moulds) are pathogenic or saprophytic non-photosynthetic Stramenopiles, mostly known for causing devastating agricultural and aquacultural diseases. Whilst molecular knowledge is restricted to crop pathogens, pathogenic oomycetes actually infect hosts from most eukaryotic lineages. Molecular evidence indicates that Eu. dicksonii belongs to the most early-branching oomycete clade known so far. Therefore Eu. dicksonii is of considerable interest due to its presumed environmental impact and phylogenetic position. Here we report the first large scale functional molecular data acquired on the most basal oomycete to date. 9873 unigenes, totalling over 3.5Mb of sequence data, were produced from Sanger-sequenced and pyrosequenced EST libraries of infected Ectocarpus siliculosus. 6787 unigenes (70%) were of algal origin, and 3086 (30%) oomycete origin. 57% of Eu. dicksonii sequences had no similarity to published sequence data, indicating that this dataset is largely unique. We were unable to positively identify sequences belonging to the RXLR and CRN groups of oomycete effectors identified in higher oomycetes, however we uncovered other unique pathogenicity factors. These included putative algal cell wall degrading enzymes, cell surface proteins, and cyclophilin-like proteins. A first look at the host response to infection has also revealed movement of the host nucleus to the site of infection as well as expression of genes responsible for strengthening the cell wall, and secretion of proteins such as protease inhibitors. We also found evidence of transcriptional reprogramming of E. siliculosus transposable elements and of a viral gene inserted in the host genome.</p

Grenville-Briggs, Laura J.

Gachon, Claire M.M

Kuepper, Frithjof C.

Swepub

A Molecular Insight into Algal-Oomycete Warfare [Elektronisk resurs] : cDNAAnalysis of <em>Ectocarpus siliculosus</em> Infected with the BasalOomycete<em> Eurychasma dicksonii</em>

Gachon, Claire M M

Kuepper, Frithjof C

The Open University

(1723) Transcriptome of Aphanomyces euteiches: new oomycete putative pathogenicity factors and metabolic pathways PLoS

A New Method for Predicting the Subcellular Localization of

A translocation signal for delivery of oomycete effector proteins into host plant cells.

Advances in research on root pathogenic oomycetes.

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Cell wall chitosaccharides are essential components and exposed patterns of the phytopathogenic oomycete Aphanomyces euteiches

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Characterisation of chitin and chitin synthase from the cellulosic cell wall fungus Saprolegnia monoica.

Chitin synthases from Saprolegnia are involved in tip growth and represent a potential target for anti-oomycete drugs.

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Clustal W and Clustal X version 2.0.

Differential host susceptibility to the marine oomycete pathogen Eurychasma dicksonii detected by real-time PCR: not all algae are equal.

Ecology of lower oomycetes.

Elevated Amino Acid Biosynthesis in Phytophthora infestans During Appressorium Formation and Potato Infection.

Engineering a software tool for gene structure prediction in higher organisms.

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Immunofluorescence and electron microscopic studies of microtubule organisation during the cell cycle of Dictyota dichotoma

Infection experiments reveal broad host ranges of Eurychasma dicksonii (Oomycota) and Chytridium polysiphoniae (Chytridiomycota), two eukaryotic parasites in marine brown algae (Phaeophyceae).

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Madan A

Massive occurrence of the heterokont and fungal parasites Anisolpidium,

Molecular basis for site-specific readout of histone H3K4Me3 by the BPTF PHD finger of NURF.

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Mutational Patterns Cannot Explain Genome Composition: Are There Any Neutral Sites in the Genomes of Bacteria?

New insights into animal pathogenic oomycetes.

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Phylogenetic affinities of two eukaryotic pathogens of marine macroalgae, Eurychasma dicksonii (Wright) Magnus and Chytridium polysiphoniae Cohn.

Plasmodium falciparum and Hyaloperonospora parasitica effector translocation motifs are functional in Phytophthora infestans.

Predicting transmembrane protein topology with a hidden Markov model: Application to complete genomes.

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Saprolegnia parasitica, an oomycete pathogen with a fishy appetite:new challenges for an old problem.

Societal costs of late blight in potato and prospects of durable resistance through cisgenic modification.

Structure of the cell walls of marine algae and ecophysiological functions of the matrix polysaccharides.

Sulfated fucans, fresh perspectives: structures, functions, and biological properties of sulfated fucans and an overview of enzymes active toward this class of polysaccharide.

The ‘‘Cheshire Cat’’ escape strategy of the coccolithophore Emiliania huxleyi in response to viral infection.

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The malarial host-targeting signal is conserved in the Irish potato famine pathogen.

The putative RxLR effector protein SpHtp1 from the fish pathogenic oomycete Saprolegnia parasitica is translocated into fish cells.

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Update: The cytoskeleton as a regulator and target of biotic interactions in plants.

YNGI PHD finger binding to H3 trimethylated at K4 promotes NuA3 HAT activity at K14 of H3 and transcription of a subset of targetted ORFs.

A molecular insight into algal-oomycete warfare : cDNA analysis of Ectocarpus siliculosus infected with the basal oomycete Eurychasma dicksonii

Brown algae are the predominant primary producers in coastal habitats, and like land plants are subject to disease and parasitism. Eurychasma dicksonii is an abundant, and probably cosmopolitan, obligate biotrophic oomycete pathogen of marine brown algae. Oomycetes (or water moulds) are pathogenic or saprophytic non-photosynthetic Stramenopiles, mostly known for causing devastating agricultural and aquacultural diseases. Whilst molecular knowledge is restricted to crop pathogens, pathogenic oomycetes actually infect hosts from most eukaryotic lineages. Molecular evidence indicates that Eu. dicksonii belongs to the most early-branching oomycete clade known so far. Therefore Eu. dicksonii is of considerable interest due to its presumed environmental impact and phylogenetic position. Here we report the first large scale functional molecular data acquired on the most basal oomycete to date. 9873 unigenes, totalling over 3.5Mb of sequence data, were produced from Sanger-sequenced and pyrosequenced EST libraries of infected Ectocarpus siliculosus. 6787 unigenes (70%) were of algal origin, and 3086 (30%) oomycete origin. 57% of Eu. dicksonii sequences had no similarity to published sequence data, indicating that this dataset is largely unique. We were unable to positively identify sequences belonging to the RXLR and CRN groups of oomycete effectors identified in higher oomycetes, however we uncovered other unique pathogenicity factors. These included putative algal cell wall degrading enzymes, cell surface proteins, and cyclophilin-like proteins. A first look at the host response to infection has also revealed movement of the host nucleus to the site of infection as well as expression of genes responsible for strengthening the cell wall, and secretion of proteins such as protease inhibitors. We also found evidence of transcriptional reprogramming of E. siliculosus transposable elements and of a viral gene inserted in the host genome.QC 2012021

Academic Archive On-line (KTH, Sweden)

A Molecular Insight into Algal-Oomycete Warfare : cDNAAnalysis of Ectocarpus siliculosus Infected with the BasalOomycete Eurychasma dicksonii

Publikationer från KTH

Digitala Vetenskapliga Arkivet - Academic Archive On-line

Gachon, Claire

Sterck, L

Küpper, Frithjof

University of the Highlands and Islands Institutional Repository

A molecular insight into algal-oomycete warfare:cDNA analysis of Ectocarpus siliculosus infected with the basal oomycete Eurychasma dicksonii

http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=3174193

A molecular insight into algal-oomycete warfare : cDNA analysis of Ectocarpus siliculosus infected with the basal oomycete Eurychasma dicksonii

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