Abdul Aziz, Saleha

Marimuthu, Murugaiyah

Mohd Noh, Nurul Shakira

Universiti Putra Malaysia Institutional Repository

Proc. 33rd MSAP Ann. Conf., 4 -7 June 2012, Langkawi, MalaysiaDETECTION OF Campy/obacter spp. IN SELECTED FARMS AROUNDSELANGOR.M. Murugaiyahl*, M. N. Shakiral and A. A. Saleh a 2lDepartment of Clinical Studies; 2Department of Veterinary and Pathology and Microbiology,Faculty Veterinary Medicine, Universiti Putra Malaysia*Email: muru@vet.upm.edu.myCampylobacter is considered as one of the emerging food and water borne zoonoticpathogens. This bacteria is a major cause of food borne diarrhoeal illness in humans.The bacteria normally inhabit the intestinal tract of warm-blooded animals and it hasbeen isolated from various species of animals and animal origin food products. Mostof the studies on detection and characterization of Campylobacter have been carriedout in developed countries whereas such data is lacking in developing countries likeMalaysia. With this scenario in view, this study was carried out to isolateCampylobacter spp. from several goats' farms around Selangor. The objective of thisstudy was to determine the prevalence and occurrence of Campylobacter species fromthe rectum of the goatSixty goats were randomly selected from three different goat farms located atHulu Langat, Bangi and TPU(Taman Pertanian Universiti), Selangor. Rectal swabswere collected to detect the presence of Campylobacter spp. The rectal swab wasplaced in a Cary Blair transport medium (oxoid) for detection of Campylobacter spp.Cool box with ice was used in order to transport the samples to the Veterinary PublicHealth Laboratory, Universiti Putra Malaysia.Rectal swab was inoculated directly onto the Campylobacter Blood-FreeSelective Agar base (CM739; Oxoid) supplemented with CCDA (Charcoal-Cefoperozine-Deoxycholate agar), Campylobacter Selective Supplement (SRI 55;Oxoid), cefoperazone 32 ug ml-1 and amphotericin B 10 ~g ml" . The plates werethen incubated at 42°C for 48 hours under microaerophilic conditions by the use of ananaerobic jar containing a gas generating pack (oxoid; 5% 02, 10% CO2, 85% N2).The positive growth plate had round, raised and convex colonies. The colonies had atendency to spread along the streak lines. Each suspected colony was then subjectedto gram staining and these bacteria had appeared as gram negative, spiral rods. Thesepresumptive colonies were subcultured on Colombia blood agar with 5% defibrinatedhorse blood. These plates were then incubated at 37°C for 48 hours in order to get thepure culture. Wet mount and gram staining were repeated on each suspected purecolony from the Colombia blood agar before proceeding to the biochemical tests. Thebiochemical tests carried were that of oxidase, catalase, Hippurate hydrolysis, Indoxylacetate hydrolysis and urease. The entire biochemical tests were all positive.Out of60 goats, 9 (15.0%) goats were positives for Campylobacter spp. Out ofthe nine isolates, 5 (55.6%) were C. jejuni and 4 (44.6%) were C. coli. The presentresult indicated that the occurrence of Campylobacter in goat as low, however theseresults are also compatible with other studies done in other countries (Salihu et al.,2009). This low detection of the limited Campylobacter spp. might be due to failureor error occurring during the procedure of the isolation of the organism. Apart fromthat, the low detection could also be due to the use of CampyGen gas generatingsystem is only suitable for growth of thermophilic Campylobacters while for non-thermophilic Campylobacters such as C. sputorum, C. consisus, C. mucosalis, C.hyointestinalis, the growth are suppressed as this system does not create hydrogen-enriched atmosphere which is important for detection of these typesO of132Proc. 33rd MSAP Ann. Conf., 4 -7 June 2012, Langkawi, MalaysiaCampylobacter. Other than that, Campylobacter also have the ability to enter intoviable but non culturable state where in this state, the pathogen response to survivalstress by not being cultured on laboratory media (Bhavsar, S. P., et al., 2007).Another possibility that affected the detection of Campylobacter spp. could be due tothe use of Modified CCDA agar which contains cefoperazine as the selectivesupplement and this does not favour growth of some Campylobacter spp.. In onestudy done by Aspinall et al., (1993), mCCD agar was designed for use at 37°C inorder to isolate C. upsaliensis as well as other thermophilic campylobacters. However,the result showed that C. upsaliensis was resistant to cefoperazina,The present study indicated that the occurrence of Campylobacter spp. in goats was15% from selected goat farms in Selangor. This result showed that there was a lowoccurrence of the organisms in goats; however the present study results are similarwith the studies done worldwide. Further studies need to be done to get the actualprevalence of Campylobacter spp.1) Aspinall, S. T., Wareing, D. R. A., Hayward, P. G., and Hutchinson, D. N.(1993).Selective medium for thermophilic campylobacters includingCampylobacter upsaliensis. J. Clin. Pathol.,46, 829-831.2) Bhavsar, S. P., and Kapadnis, B. P. (2007): Virulence factors of Campylobacter.The Internet Journal of Microbiology. 2007 3(2).3) Salihu, M.D., Junaidu, A. U., Oboegbulem, S. I., Egwu, G. 0., Tambuwal, F. M.,and Yakuba, Y. (2009). Prevalence of Campylobacter species in apparentlyhealthy goats in Sokoto state (Northwestern) NigeriaAfr. J. Microbio!. Res., 3(9):572-574.133

Detection of Campylobacter spp. in selected farms around Selangor

http://psasir.upm.edu.my/50859/1/DETECTION%20OF%20Campyobacter%20spp.%20IN%20SELECTED%20FARMS%20AROUND.pdf

Detection of Campylobacter spp. in selected farms around Selangor

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