Chia, M. L.

Kumar, S. V.

Panandam, Jothi Malar

Regina, V. I.

Universiti Putra Malaysia Institutional Repository

UPM Research Report 1998: Volume 2GENETIC EVALUATION OF SOME SHEEP BREEDS1.M. Panandam, S.V. Kumar, V.I. Regina andM.L. Chia 'Faculty of AgricultureUniversiti Putra Malaysia, 43400 UPM, Serdang, Selangor,MalaysiaKeywords: sheep, Cervus timorensis, polymorphism,RAPD, isoenzymes.IntroductionGeneral improvement of genetic resources using selectionand crossbreeding depends on genetic variability. In recentyears, Malaysia has imported many new sheep breeds and alarge number of deer from various sources (Rajion et al.1993). Little is known of the genetic background of theseanimals. This project was initiated to evaluate the geneticvariability within and between some breeds of sheep andsome deer populations in the country using biochemical andmolecular polymorphism techniques.Materials and MethodsSix sheep breeds, namely the Bali Bali (BB), BarbadosBlackbelly (BBb), Dorset-Malin cross (DM), Malin (M),Santa Ines (SI) and St. Croix (SC), and 2 populations of therusa deer (Cervus timorensis) of different origins (from NewCaledonia and Mauritius) were analysed for molecular andbiochemical polymorphism. Blood samples from 30 animalsfrom each population were analysed for molecular markersusing the random amplified polymorphic DNA (RAPD)technique. Eight 10-mer oligonucleotide primers of arbitrarysequence were used to generate the RAPD data for 4 of thesheep breeds and two 12-mer primers were used for the deer.198Samples from 50 animals per population were analysed forbiochemical markers using cellulose acetate electrophoresis(CAE). Nine markers were analysed for three of the sheepbreeds and eight for the deer populations.Results and DiscussionRAPD analysis of the sheep breeds generated 7 - 11 bandsper primer and a total of 65 markers. One of the primers de-tected no polymorphism (OPD-09) while percent polymor-phism for the others ranged between 36.36 - 100 %. Thebiochemical study showed haemoglobin, nucleoside phos-phorylase and glucose phosphate isomerase to be polymor-phic. Percent polymorphism for BB, SI and SC were 33.33,22.22 and 11.11 %, respectively. RAPD analysis of the twodeer populations also generated 7 - 11 bands per primer.Percent polymorphism for primer 06B was 83.3 % while thatfor primer 52A was 66.66 %. The biochemical study showedhaemoglobin, malate dehydrogenase and malic enzyme to bepolymorphic. Percent polymorphism was 37.5 % for the twopopulations.ConclusionsAs expected the molecular study was able to detect highervaria~~n than the biochemical study. Work on analysing theremrum.ngshe~p breeds and deer populations are continuing.More biochemical markers are also being investigated.ReferencesRajion, M.;-\., Alimon, .A.R., Davis, M.P., 1993. Goats and sheepproduction. In: Ammal Industry in Malaysia, Eds. Fatimah,C.T.~:I.,Ramlah,.A.H. and Bahaman A.R. Faculty of VeterinaryMedicine and Animal Science, Universiti Pertanian Malaysia, p.51-67.Supported by IRPA Grant09-02-04-0042

Genetic evaluation of some sheep breeds

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Genetic evaluation of some sheep breeds

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