Scurrula parasitica (Loranthaceae) is a widespread aerial hemiparasitic plant in southwest China, recognized for its ecological roles and broad host range. As a representative of mistletoes in Santalales, it serves as a model for studying the genomic basis of aerial hemiparasitism. Here, we present a high-quality chromosome-level genome assembly of S. parasitica using PacBio high-fidelity and Hi-C sequencing. The assembled genome spans 547.41 Mb with a contig N50 of 8.32 Mb, and 97.54% of the sequence is anchored to nine pseudochromosomes. Repetitive sequences account for 64.53% of the genome. We predicted 21,837 protein-coding genes, of which 20,974 (96.05%) received functional annotations.Additionally, we identified 1,271 transcription factor genes and 8,407 non-coding RNAs. This chromosome-level assembly provides a foundational resource for investigating gene family evolution, parasitic adaptation, and genome architecture in S. parasitica. The genome assembly and associated datasets have been deposited in public repositories, enabling future comparative and functional genomic studies in parasitic angiosperms
