CORE
🇺🇦
make metadata, not war
Services
Services overview
Explore all CORE services
Access to raw data
API
Dataset
FastSync
Content discovery
Recommender
Discovery
OAI identifiers
OAI Resolver
Managing content
Dashboard
Bespoke contracts
Consultancy services
Support us
Support us
Membership
Sponsorship
Community governance
Advisory Board
Board of supporters
Research network
About
About us
Our mission
Team
Blog
FAQs
Contact us
research
Development and partial characterization of new marine cell line from brain of Asian sea bass Lates calcarifer for virus isolation.
Authors
Azila A. Abdullah
Siti Suri Arshad
+3 more
Mohd Hair Bejo
Mauida F. Hasoon
Hassan Mohd Daud
Publication date
17 November 2010
Publisher
'Springer Science and Business Media LLC'
Doi
Abstract
A new cell line, Asian sea bass brain (ASBB), was derived from the brain tissue of Asian sea bass Lates calcarifer. This cell line was maintained in Leibovitz L-15 media supplemented with 10% fetal bovine serum (FBS). The ASBB cell line was subcultured more than 60 times over a period of 15 mo. The ASBB cell line consists predominantly of fibroblastic-like cells and was able to grow at temperatures between 20°C and 30°C with an optimum temperature of 25°C. The growth rate of these cells increased as the proportion of FBS increased from 2% to 20% at 25°C with optimum growth at the concentrations of 10% or 15% FBS. Polymerase chain reaction products were obtained from ASBB cells and tissues of sea bass with primer sets of microsatellite markers of sea bass. An isolate of piscine nodavirus from juveniles of marine fish species tested positive by IQ2000 kit for viral nervous necrosis detection and was examined for its infectivity to a fish cell line of ASBB. A marine fish betanodavirus was tested to determine the susceptibility of this new cell line in comparison with commercial highly permissive SSN-1 cells. The ASBB cell line was found to be susceptible to nodavirus (RGNNV genotype), and the infection was confirmed by comparison cytopathic effect (CPE) with commercial SSN-1 and reverse transcriptase-polymerase chain reaction. A nodavirus was further elucidated by electron microscopy, and the virus tested was shown to induce CPE on ASBB cells with significant high titer. This suggests that the ASBB cell line has good potential for the isolation of fish viruses. © 2010 The Society for In Vitro Biology
Similar works
Full text
Open in the Core reader
Download PDF
Available Versions
Universiti Putra Malaysia Institutional Repository
See this paper in CORE
Go to the repository landing page
Download from data provider
oai:psasir.upm.edu.my:25409
Last time updated on 08/04/2018
Universiti Putra Malaysia Institutional Repository
See this paper in CORE
Go to the repository landing page
Download from data provider
oai:psasir.upm.edu.my:9364
Last time updated on 03/08/2016
Crossref
See this paper in CORE
Go to the repository landing page
Download from data provider
info:doi/10.1007%2Fs11626-010-...
Last time updated on 29/04/2021