Caribbean reefs face increasingly frequent and intense bleaching events, adding to the numerous other threats impacting these ecosystems. Addressing these challenges requires global action to reduce climate drivers, along with local efforts like reef restoration. Active restoration using thermotolerant coral colonies offers a potential strategy to alleviate these impacts; however, gaps remain in identifying context-specific temperature thresholds to guide colony selection and standardize thermotolerance assessment methods. This study addressed these gaps in two phases. First, by determining practical thresholds to differentiate species responses to heat stress; and second, by developing a framework to identify and prioritize resilient colonies for restoration. In the first phase, 70 colonies of Acropora cervicornis, Diploria labyrinthiformis, Montastraea cavernosa, Orbicella annularis, O. faveolata, Porites astreoides, and P. porites were sampled from reefs in the southeastern Dominican Republic. Heat stress responses were assessed through 3-hour heat pulse assays above the local maximum monthly mean (MMM) temperature, combining visual bleaching ranks, pixel intensity as a proxy for chlorophyll loss, and pulse amplitude modulated (PAM) fluorometry. Species-specific T50 thresholds were identified as the temperatures where 50% of colonies showed signs of stress. In the second phase, intraspecific thermotolerance was further examined for D. labyrinthiformis, M. cavernosa, O. annularis, O. faveolata, and P. astreoides using 99 colonies from known parent sources. Heat pulse assays at control (MMM) and T50 temperatures were repeated four times to assign colony-specific thermal performance scores. This study integrates inter- and intraspecific thermotolerance data into a practical selection framework, offering valuable insights to guide restoration under climate change.<br/
