A simple, automated manometric method is here discussed and applied to perform batch experiments for the stoichiometric and kinetic characterization of biological denitrification. The main strength of the proposed methodology is that it requires simple instrumentation, which is usually available in wastewater treatment plant laboratories, being it used in BOD and in BMP tests. The experimental setup consists of a glass bottle, a mixing and termostated system and a manometric bottle-head which can measure and log the overpressure that is caused by denitrified N2. At first, tests were conducted to determine the repeatability of the method; they were performed under low Food-to-Biomass ratio and with both endogenous and externally dosed carbon sources. Later, experimental procedures were performed to assess (1) the anoxic growth yield, (2) the endogenous anoxic decay rate; (3) the anoxic growth rate on acetate; (4) the fraction of anoxic active biomass in the sludge sample. Sludge samples for all these tests were taken from two WWTPs and 6 to 10 replicates were performed each time. Results indicated that the testing procedures is well repeatable and reliable and resulting estimates were within reported literature values
