Contamination is likely to affect the landscape composition, usually linked to ecological fragmentation, which may impact the distribution, persistence and abundance of species. In the current study, the estuarine shrimp Palaemon varians was exposed to copper (25 and 0.5 μg/L) simultaneously to predation signal and food to evaluate the organism’s spatial distribution within a spatially heterogeneous landscape. All experimental landscapes were simulated in the Heterogeneous Multi Habitat Assay System (HeMHAS). As results, P. varians detected and avoided copper, however, predation signal shifted the response to preference over regions with conditions previously avoided, even if that meant to increase copper exposure. When confronted to move towards environments with high food availability, lower connectivity occurred among the shrimps’ populations isolated by contamination and predation risk simultaneously. This indicate that contamination might: (i) trigger avoidance in shrimps, (ii) prevent the colonisation towards foraging areas, (iii) enhance the populations’ isolation and (iv) make populations more susceptible to local extinction