Adelani, Isaacson

Bankole, G.E.

Rotimi, Oluwakemi A

Rotimi, S. O

Covenant University Repository

  Nutrition Effects of Naringin on hepatic paraoxonase activity and lipid metabolism in type 2 diabetic rats Solomon Oladapo Rotimi ,  Isaacson B Adelani ,  Goodness Esther Bankole , and  Oluwakemi A Rotimi Published Online:1 Apr 2016Abstract Number:692.19 •  •  Abstract Naringin is one of the citrus-derived flavonoids that have been reported for its antihyperglycemic, antihyperlipidemic and antioxidant properties. Although its usefulness as nutraceutical in the management of diabetes has been reported, there is a dearth of information on involvement of lipid-associated protein antioxidant like paraoxonase in its antidiabetic activity. In order to investigate this, high fat-low streptozocin model of type 2 diabetic rats were treated daily with 50mg/kg, 100mg/kg and 200mg/kg naringin orally for 21days. The levels of plasma insulin and dipeptidyl peptidase-4 (DPP IV) were determined using enzyme-linked immunosorbent assay while other biomarkers of T2DM, activity of paraoxonase and lipid profile were assayed spectrophotometrically. The levels of expression of hepatic 3-hydroxy-3-methyl-glutaryl-CoA reductase (HMGCR), scavenger receptor class B member 1 (SCARB1), Aryl Hydrocarbon Receptor (AhR), Lipoprotein Lipase (LIPL), and Lecithin—cholesterol acyltransferase (LCAT) were assessed using reverse transcriptase polymerase chain reaction technique. Naringin treatment resulted in a significant (p<0.05) decrease in levels of plasma glucose, insulin, free fatty acid, amylase and DPP IV. Naringin treatment was also associated with a significant (p<0.05) decrease in the levels of cholesterol and triglyceride in the plasma and liver in the dose-dependent pattern. In the liver, the activity of carnitine palmitoyltransferase was significantly increased (p<0.05) while the expression of HMGCR was only significantly (p<0.05) reduced by 200mg/Kg naringin treatment. Meanwhile, SCARB1, AhR, LIPL and LCAT were significantly (p<0.05) upregulated by naringin treatment. Although T2DM resulted in increased activities of PON in the plasma, HDL and VLDL, a decrease in activity was observed in the liver. These alterations in PON activities were however significantly (p<0.05) reversed by naringin treatment. These results showed that PON is involved in the antidiabetic action of naringin. •  Vol. 30, No. 1_supplement April 2016  Metrics Downloaded 0 times  Publication History  Published online 1 April 2016 •  9650 Rockville Pike Bethesda, MD 20814 301-634-7000 © 2018 by the Federation of American Societies for Experimental Biology   

Effects of Naringin on hepatic paraoxonase activity and lipid metabolism in type 2 diabetic rats

http://eprints.covenantuniversity.edu.ng/10482/1/Effects%20of%20Naringin.pdf

Effects of Naringin on hepatic paraoxonase activity and lipid metabolism in type 2 diabetic rats

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