CESARO, ELENA

COSTANZO, PAOLA

CRESCITELLI R.

FATTORE L.

FLORIO F.

IZZO, PAOLA

MONTANO, GIORGIA

English

Archivio della ricerca - Università degli studi di Napoli Federico II

The Kruppel like zinc finger protein, ZNF224, is a novel transcriptional co-regulator of WT1Montano G., Florio F., Cesaro E., Crescitelli R., Fattore L., Izzo P., Costanzo P.Dipartimento di Biochimica e Biotecnologie Mediche, Università degli Studi di Napoli “Federico II”.Wilms’ tumour 1 protein, WT1, is a zinc finger transcriptional factor that controls the expression of a wide number of genes involved in tissue differentiation, in apoptosis, or in cell cycle control. WT1 gene encodes for two major splicing isoforms participating in both transcriptional and post-transcriptional gene regulation: WT1(-KTS) is the “transcriptional isoform” and WT1(+KTS) is the “post-transcriptional isoform”, arising from the exclusion or insertion of amino acids lysine, threonine and serine (KTS) between the third and fourth zinc fingers, respectively.  Moreover, the transcriptional regulatory properties of WT1 are known to be influenced by the interaction with an increasing number of molecular partners. In this study, we identify the Krüppel-like zinc finger protein, ZNF224, as a novel WT1-associating protein and demonstrate that ZNF224 may act as a transcriptional co-regulator of WT1 to modulate the expression of apoptotic genes. By co-immunoprecipitation assay we demonstrate that ZNF224 specifically interacts with the WT1 (-KTS) isoform and that this interaction is only present in the nucleus. Knockdown of ZNF224 in the human erythroleukemia cell line K562 affects the expression of WT1 apoptotic target genes, supporting a role for ZNF224/WT1 interaction in the transcriptional control  of apoptosis.Biochimica cellulare

The Kruppel like zinc finger protein,ZNF224, is a novel trascriptional co-regulator of WT1

https://www.iris.unina.it/bitstream/11588/372086/1/abstract%20Milano%20SIB%202010.doc

The Kruppel like zinc finger protein,ZNF224, is a novel trascriptional co-regulator of WT1

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Archivio della ricerca - Università degli studi di Napoli Federico II