Comparison of PCR-based DNA markers for using different Lr19 and Lr24 leaf rust resistance wheat sources

Abstract

Both resistance genes Lr19 and Lr24 originate from Agropyron elongatum . The gene Lr24 is derived from two different translocations: 1BS/3Ag (‘Amigo’) or 3DS/3Ag (‘Agent’). The use of molecular markers makes selection easier during the breeding process as well as in the selection of the parents. In this study, two markers were used to identify the gene Lr19 (GbF/R 130 , SCS265 512 ) and four different markers (J9/1-2 310 , SC-H5 700 , SCS1302 613 and SCS1326 607 ) were available to search for the gene Lr24 . The GbF/ R130 marker for gene Lr19 worked well, but the SCAR marker SCS265 512 proved to be easier to use in MAS. SCAR markers SCS1302 613 and SCS1326 607 proved to be highly reliable and effective for gene Lr24 not only in Agent-derived sources but also in ‘Amigo’ derivatives. The STS marker J9/1-2 310 and the SCAR marker SC-H5 700 required several modifications and were effective only in ‘Agent’ offsprings