St. Petersburg branch of the Russian Association of Allergologists and Clinical Immunologists
Doi
Abstract
AbstractMalignant neoplasms often arise against the background of immunodeficiency. But anticancer chemotherapeutic drugs can also cause the development or aggravate immunodeficiency, affecting the bone marrow function. Previous studies have shown that the bioorganocomplex obtained from the chickens’ Fabricius bursa has an antioxidant and immunotropic effect. The aim of this research was to investigate the protective mechanism of the bioorganocomplex «Bursanatal» against cyclophosphamide-induced immunosuppression in the bone marrow, thymus, spleen of C57Bl/6 mice. Immunodeficiency was induced with single cyclophosphamide injection (200 mg/kg). Histological, morphometric, radiological methods were used, as well as immunohistochemical staining of the spleen and thymus with CD45 and CD3 antibodies. The studies showed that cyclophosphamide injection affects both peripheral blood and bone marrow. Moreover, neutrophilic, basophilic and erythroid precursor cells are less sensitive to the cyclophosphamide. So on the 8th day their compensatory hyperplasia is observed. The use of the bioorganocomplex is accompanied by the leukocytes, erythrocytes and hemoglobin increase in the peripheral blood. The cellularity of the bone marrow increases due to the dividing and maturation of erythrocyte, neutrophil, basophil and lymphocyte lineage cells. Bioorganocomplex administration causes a reliable decrease the area of the red pulp in spleen of mice with cyclophosphamide-induced immunodeficiency. The activation of extramedullary hematopoiesis are found in the spleen of treated mice – the number of colony-forming hematopoietic progenitors increases. Also it is noted that the area of the reactive center in the white pulp decreases with a significant increase the CD3+ cells in the red and white pulp under the «Bursanatal» treatment. At the same time, it does not affect thymus pathomorphology
