Discovenes on the efficacy of antiretroviral drug have changed the management of HIV patients. More information on viral load is needed with accurate markers of remission and/or eradication. First objective: to evaluete the sensitivity of an innovative protocol for p24 antigen(3 Plus). The p24 assay has a sensitivity of 70 fg/reaction correlates with viral replication,viral load,CD4+cell numbers and therapy efficacy. Thuss it is a useful and inexpensive marker in AIDS management. Second objective: to develop and evaluate assays for total HIV DNA and non-integrated circular DNA. Total HIV DNA was detected in 96.9% of 548 samples from HIV-infected individuals. High levels of total HIV DNA in sequential samples correlated with therapy inefficacy and subsequent HIV replication,confirmed by the detection of non-integrated DNA. In absence of NI-DNA, total HIV DNA contrains only proviral DNA and is the only marker of remission and/or eradication
