Sutiman B, Sumitro

Sutini, -

Tatik, W

Verporte, R

English

UPN Jatim Repository

                                                                                                                                   573                                   ISNAR C2FS PROCEEDING Natural Resources Climate Change and Food Security in Developing Countries Surabaya, Indonesia, June 27-28, 2011  Sub Theme      : Adaptation and Production                                                Oral Presentation ISBN                :  978-602-8915-93-9  O  HO OH O  OH O OH OH OH Figure 1. EGCG structure (Thomson, 2004).  CHARACTERIZATION OF EGCG COMPOUND USE 1H NMR SPECTRUM ON CAMELLIA  SINENSIS (L.) CALLUS   Sutini 1, Tatik W2, Sutiman B3, R. Verpoorte 4  1Agrotecnology Department of Agriculture Faculty UPN ‘Veteran’, Surabaya-East Java.           2 Agronomy Department of Agriculture Faculty, Brawijaya University, Malang-East Java 3 Biology Department of FMIPA, Brawijaya University, Malang-East Java 4Plant Metabolomics Department - Leiden University.  Netherlands Email : tien_basuki@yahoo.com   ABSTRACT Epigallocatechin gallate (EGCG) are secondary metabolite on Camellia sinensis L as obesity preventing agent. The characterisation of this plant use 1H NMR spectroscopy often have been done, however characterisation on callus both drying with open air and without drying is rare. The purpose of this research is characterize EGCG of tea callus via process both drying with open air and vacuum. Tis method use 1H NMR spectroscopy. The result show that EGCG character of tea callus via process both drying with open air and vacuum are significantly different.  Key note: Epigallocatechin gallate, 1H  NMR,  Camellia sinensis L callus  INTRODUCTION Epigallocatechin gallate (EGCG) bioactive is available on tea (Camellia sinensis L). The advantage of this are anti obesity, anti cancer, anti diabetic, anti cholesterol, anti bakterial, cardiovaskuler disease and osteoporosis prevention agent. Many function of tea on industry that is beverage, cosmetic, pharmacy, and food (Hartoyo, 2003).  EGCG bioactive compound structure as figure 1, is one of flavonoid derivat of phenol on tea (Camellia sinensis L.). Structure that have many hydroxi/ OH- easy to bond free radical so EGCG identified have multi function in health.                                                                                                                                            574                                   ISNAR C2FS PROCEEDING Natural Resources Climate Change and Food Security in Developing Countries Surabaya, Indonesia, June 27-28, 2011  Sub Theme      : Adaptation and Production                                                Oral Presentation ISBN                :  978-602-8915-93-9  Peter W.L. (2000) say that EGCG harvested on winter and summer season different on EGCG value.   This is relevan with Caffin, N., D’Arcy, B.,Yao L.,Rintou, N. (2004) note that EGCG amount of tea leaves is increasing harvested on summer (May), however decreasing on winter (November).  In general, this research is aimed at developing production of EGCG technology in  vitro by callus culture technique. Characteristics of EGCG are: binding with several biologic matrix and heavy metals, catalyzing electron transportation, and trapping free radicals. Four characteristics above made it a bioactive agent. Hence secondary metabolite of EGCG must be characterized with 1H NMR  both on drying and undrying process to improve the product quality.  :  Purpose  : Characterize EGCG of tea callus with process both drying in open air and with vacum.   MATERIALS AND METHOD 1H NMR spectroscopy 500 MHz (Bruker,Jerman),  1.5 mL-ependorff tube mL -2 mL,  5 mm NMR tube,  centrifuge, Ultrasonic, vortex, vacum dry, metanol-deuterium  (CH3OH-d4), buffer KH2PO4 in D2O (pH 6,0) containing 0,01% (b / b) TSP, aqua bidestilata. Extraction Preparing metanol-deuterium  (CH3OH-d4) without add standar internal,  buffer KH2PO4 in D2O (pH 6,0) containing 0,01% (b / b) TSP. Measure gentle powder of 25-50 mg tea callus that both with drying in open air and vacum.   Then add  CH3OH-d4 (without any internal standard), KH2PO4 buffer in D2O (pH 6.0) containing 0.1% (w/w) TSP, in to 2 mL-ependorff tube.  This solution vortex for 1 minute at room temperature and then ultrasonication for 5-20 minute at room temperature. This solution centrifuge at room temperature for 5 – 20 minute using microtube centrifugator (13000 rpm, room temperature). Transfer supernatant (more than 1 mL) to 1.5 mL-ependorff tube. If more centrifugation is necessary centrifugator using microtube centrifugator (13000 rpm, 1 minute, room temperature). Then transfer 800 mL of supernatant to 5 mm NMR tube.                                                                                                                                      575                                   ISNAR C2FS PROCEEDING Natural Resources Climate Change and Food Security in Developing Countries Surabaya, Indonesia, June 27-28, 2011  Sub Theme      : Adaptation and Production                                                Oral Presentation ISBN                :  978-602-8915-93-9  Characterisation Use 1H Nmr The study done use 500 spectrometer  MHz 1H NMR (Bruker,Jerman) completed by cryoprobes. Chemical shift(δ)  is measured on ppm, with standart referency use tetrametil silen zero ppm, with chemical shift range between 4.52-7.08.   RESULTS AND DISCUSSION  The liquid of green browny pure extraction, then spectrum observed.  EGCG spectra of tea callus proceed both with drying in open air and wet tea callus in vacumand standar as Figure 1. Spectrum 1H NMR 500 MHz on methanol deuteurium solvent, (Table 1) showed that  chemical shift (δ ppm) and space between two spin/kopling constanta (J in Hz). Proton position are structure from EGCG resonance on H-6, H-8, H-2’, H-5’,H-6’ (Markam, et al, 1994).  Chemical shift and coupling constanta on proton position  resonance for EGCG  show that  tea callus with drying in vacum almostly same with standart.  This show that tea callus characterization with drying vacum can identify EGCG character, that not happen in tea callus with drying in open air.  Proton position of EGCG resonance on chemical shift (δ) and coupling constanta (J), tea callus with open air drying can not show the character because that compound oxydated by air.  Based on Nathalie V, G. research (2001) that flavonoid oxydation caused by temperatur, UV light, and ion Cu2+ then change to be unstabile quinon into sulfonat.  Using 1H NMR spectroscopy,  can characterize proton of EGCG. This is relevant to Moco research (2007) show that 1H NMR can identify flavonoid compound on tomato plant.  Then, Tarachiwin L. Et al., 2007 note that 1H NMR spectroscopy combined with  multivariat analysis can descript secondary metabolit profile.  However using 1H NMR spectroscopy, have disavantage that is: 1). Relatively low sensitivity than using other analysis technique such as MS, 2). Can produce more than one ambiguous spectra, 3). Chemical shift influenced by the surrounding chemical environment. There is many ways to solve that is: 1). Combine 2D spectrum (two dimension) NMR, 2). This research use standart data comparation refer to the same sovent material.                                                                                                                                        576                                   ISNAR C2FS PROCEEDING Natural Resources Climate Change and Food Security in Developing Countries Surabaya, Indonesia, June 27-28, 2011  Sub Theme      : Adaptation and Production                                                Oral Presentation ISBN                :  978-602-8915-93-9                                 Proton Position δEGCG tea callus drying in open air, (J in Hz) δEGCG  tea callusdrying with vacum,  (J in Hz) δEGCG  with standart  ( J in Hz) H-2 - 4.90 (s) 4.90 (s) H-3 - 5.50 (s) 5.51 (s) H-4α - 2.97 (dd ) 2.97 (dd ) H-4β - 2.83 (dd) 2.83 (dd) H-6 - 5.94(s,1.79) 5.93(s,1.79) H-8 - 5.94(s,1.79) 5.93(s,1.79) H-2’ 6.34(s) 6.49(s,1.80) 6.48(s,1.80) H-5’ - - - H-6’ 6.40(s,) 6.49(s,1.80) 6.48(s,1.80) H-2” - 6.95(s) 6.93 (s)  Table 1.   Proton position δ and J EGCG , tea callus drying in open air, tea callus                    Drying  with vacuum and standart  Figure 1. Spektrum 1H NMR 500 MHz on CH3OH-d4 solvent from: (A)  tea callus with                   vacum drying, (B), standart , (C) tea callus  with drying in open air.  A B C                                                                                                                                    577                                   ISNAR C2FS PROCEEDING Natural Resources Climate Change and Food Security in Developing Countries Surabaya, Indonesia, June 27-28, 2011  Sub Theme      : Adaptation and Production                                                Oral Presentation ISBN                :  978-602-8915-93-9  CONCLUSION Achieve character from EGCG body   tat is 1H NMR as H-6, H-8, H-2’, H-5’, H-6’ (Markam,et al, 1994). Observation on chemical shift located between 5,94 – 6,49, this value based on existence range of EGCG compound. This relevant with study by McLeod (2010) that aromatic bonding area located on 5,8 – 8,8 ACKNOWLEDGEMENT : Thanks to Prof. Dr. Rob. Verpoorte and staff  of Department Plant Metabolomics, Leiden University, Netherlands  for help and attention. REFERENCES  Caffin, N., dkk., 2004. Developing an index of quality for Australian tea, Rural Industries Research and Development Corporation, http://www.rirdc.gov.au  Hartoyo Arif. 2003. Teh dan kasiatnya bagi kesehatan. Sebuah tinjauan ilmiah, Kanisius. Yogyakarta.   Markam, dkk., 1994. H. Nuclear magnetic resonance spectroscopy of flavonoids and their glycosides inheksadeutterodimethylsulfoxide. Di dalam Harborn J.B., The flavonoid in research Science, chapman and Hall limited london.  McLeod. 2010.  New Trend Research on Eco-frienly technology for medic  and Agriculture. Basic science national seminutear. Universitas brawijaya malang.  Moço Sofia. 2007. Metabolomics Technologies applied to the Identification of Compounds in Plants. Dissertation. Wageningen Universiteit. Netherlands.  Nathalie V, G.  dkk., 2001.  Study  and quantification of  monomeric flavan-3-ol and dimeric oxidation J. Sci food Agric (81): p 1172-1179   Peter W.L. 2002. Biochemical analysis for identification of quality in black tea (Camellia sinensis), Disertasi. Faculty of Natural and Agricultural Sciences. Departement of Biochemistry. University of Pretoria. Petroria. South Africa. http://upetd.up.ac.za/thesis/available/etd-03012005 084935/unrestricted/00dissertation.pdf  Tarachiwin L. dkk., 2007. 1H NMR Based Metabolic Profiling in the Evaluation of Japanese Green Tea Quality. J. Agric. Food Chem.(55): p. 9330–9336.  Thomson Leonor. 2004. Enrichment of Biologically Active Compounds from Selected Plants Using Adsorptive Bubble Separation, Dissertation FakultätWissenschaftszentrum Weihenstephan für Ernährung, Landnutzung und Umwelt Technischen Universität München. http://www.google.co.id/search?hl=id&q=production+catechin++from+callus+camellia+sinensis+leaf+pdf+%2Bfree+purchase+research+&meta.                                                                                                                                      578                                   ISNAR C2FS PROCEEDING Natural Resources Climate Change and Food Security in Developing Countries Surabaya, Indonesia, June 27-28, 2011  Sub Theme      : Adaptation and Production                                                Oral Presentation ISBN                :  978-602-8915-93-9  Question   :  1. What the structure resonance in EGCG ? Answer      :  1. Aquired characteristic from EGCG structure resonance on H that H – 6, H – 8, H - 2, H – 5, H – 7, etc.                     

CHARACTERIZATION OF EGCG COMPOUND USE 1H NMR SPECTRUM

ON CAMELLIA SINENSIS (L.) CALLUS

http://eprints.upnjatim.ac.id/7849/1/isnar%2D2011.pdf

CHARACTERIZATION OF EGCG COMPOUND USE 1H NMR SPECTRUM ON CAMELLIA SINENSIS (L.) CALLUS

Abstract

Similar works

Full text

Available Versions

UPN Jatim Repository