Editorial Office of Journal of Guangxi Medical University
Doi
Abstract
Objective To investigate the effects of deltamethrin (DM) subchronic exposure on hepatic lipid metabolism in male mice. Methods Eighteen specific pathogen-free (SPF) male C57BL/6J mice (8-week-old) were randomly assigned to a control group (pure corn oil), a low-dose DM group (2.25 mg/kg), and a high-dose DM group (9.0 mg/kg), followed by oral gavage for 90 consecutive days. Hepatic morphological changes were observed via hematoxylin-eosin (HE) staining and Masson staining. The activities of alanine aminotransferase (ALT), aspartate aminotransferase (AST), and superoxide dismutase (SOD), as well as the levels of total cholesterol (T-CHO), triglycerides (TG), reduced glutathione (GSH), and malondialdehyde (MDA) in the liver were measured. Western blotting analysis was performed to detect the expression levels of sterol regulatory elementbinding protein-1c (SREBP-1c) and peroxisome proliferator-activated receptor α (PPARα). Results Compared with the control group, high-dose DM group showed significantly increased body weight and white adipose coefficient (P < 0.05). HE staining revealed focal inflammatory cell aggregation in liver tissues of high-dose DM group. Masson staining showed no significant fibrosis changes in any of DM dose groups. The liver ALT activity was increased in the low-dose and high-dose DM groups, and the AST activity was increased in the high-dose DM group (all P < 0.05). Compared with the control group, the level of cellular oxidative stress in the high-dose DM group was significantly increased (P < 0.05). Compared with the control group, the levels of hepatic T-CHO and TG in the high-dose DM group were increased (P < 0.05). The expression of SREBP-1c was up-regulated and the expression of PPARα was down-regulated in all DM dose groups (all P < 0.05). Conclusion Sub-chronic DM exposure induces hepatic lipid metabolic disorder in mice, possibly through oxidative stress injury and imbalanced lipid synthesis-catabolism
