Optimal culture method to improve the productivity of Haematococcus sp.KORDI03

Abstract

Haematococcus sp. are well known for the ability to synthesize and accumulate of the red ketocarotenoid astaxanthin (3,39-dihydroxy-b,b-caratene-4,49-dione) for up to 4% of the total cellular dry weight under induced stress condition (nutrient deprivation, increased salinity and exposure to high temperature). Astaxanthin is a high value-added product with applications in pharmaceuicals, nutraceuticals, and animal nutrition. However, the culture cost (nutrients, water, land and system etc.) are considerably high, it is essential to develop an affordable culture condition for mass production and sustainability of the strain. In this reason, the purpose of this study was to develop for reducing culture cost by invented culture method and to examine an optimal condition for biomass production of the Haematococcus sp. KORDI03 (KCTC 12348BP). The broth-cultured strain (3.2x106 cells/mL, 0.0012 g/L) was inoculated by different conditions of inoculationvolume and spray feed interval on BBM medium-based plates (Ø90 x 25 x duplicate) with room temperature (28±0.5°C) and fluorescence light (12L: 12D cycle) conditions during a month. Red cyst cells were observed from 10 days after inoculation (over 50% confluence), and the final production of dried red cyst cells were ranged from 1.63 g/m2 (5 mL inoculation vol. and 5 day-fed interval) to 21.12 g/m2 (1 mL inoculation vol. and 1 day-fed interval).2