Barbara, Maurizio

Battaglione, Ezio

Covelli, Edoardo

Familiari, Giuseppe

Miglietta, Selenia

Relucenti, Michela

Learning Objectives: To shed some light on the erosive processes
that affect the incus in respect to the cholesteatoma
localization.
Background: The degree of invasiveness of a cholesteatoma
is usually based on some clinical features, such as
its extension, relapsing tendency and erosive capacity.
For this latter, the ossicular chain, and mostly incus, is
usually involved, leading the surgeon to its accurate
cleansing or removal.
Material and Methods: For this study, incus specimens were
collected during tympanomastoid surgery from adult patients
with cholesteatomatous otitis media. The samples were processed
for scanning electron microscopy and the investigation
aimed to consider at first the whole bone, then to give
a detailed mapping of the eroded parts of the incus adjacent
to the cholesteatoma tissue. The different degrees of erosion
(in terms of presence/absence of erosion, lacunae and their
diameter and depth) and the presence/absence of biofilm
were considered. Erosion degree was recorded with 0 if
absent, and with 1–2–3 if mild, moderate or severe, respectively.
Five consecutive fields at 100X magnification, aligned
in 3 raws, the first one proximal and the last one distal to the
surgical erosive point were analized. A total of 60 fields for
each raw were observed.
Results: Erosion lacunae were clustered on the surface of the
eroded areas, their diameter being 75 ± 15μm. Although a
proximal to distal gradient exists, looking to the distribution
of the eroded areas, grade 3 erosion was not only limited to
the area proximal to the ossicle erosive edge (first raw) but
also in raw 2 and sometimes scattered up to raw 3. Grade 3
erosion was observed around nutrient foramina of the bone
(65%).
Discussion: Our observations confirm the hypothesis that the
erosion degree is higher near the resection edge, but also
prove that erosion areas of degree 3 can also be observed
in regions far from the erosive point

BARBARA, Maurizio

MIGLIETTA, SELENIA

RELUCENTI, Michela

BATTAGLIONE, Ezio

FAMILIARI, Giuseppe

COVELLI, EDOARDO

Archivio della ricerca- Università di Roma La Sapienza

doi:10.1017/S0022215116005041ID: IP008The symptoms and prognosis of traumaticpneumolabyrinth by air locationPresenting Author: MOO JIN BaekMoo Jin Baek1, Dong Hyun Lee2, Eui Kyeong Bang31Haeundae Paik Hospital, Inje Univsersity,Busan, Korea, 2Haeundae paik Hospital,Busan, Korea, 3Busan vetrans Hospital, Busan,KoreaLearning Objectives:Objective: The Pneumolabyrinth is a rare condition in whichair is present in the inner ear due to abnormal pathwaysbetween the middle ear and inner ear. This condition canbe caused by congenital reasons, middle ear surgery, headtrauma. The cases of traumatic pneumolabyrinth is increas-ing due to the high resolution CT. But Symptoms and prog-nosis of traumatic pneumolabyrinth is not clarified yet andneeds further investigation.Methods: We reviewed 149 cases of head trauma Patientswho underwent temporal bone CT between Jan 1st 2012 toJan 1st 2014. Review of records was done according to thefactors: temporal bone fracture, otic capsule involvement,location of air bubble, symptom improvement.Results: Ten patients showed pneumolabyrinth with symp-toms of dizziness and hearing loss. Dizziness which isrelated to air bubble in the vestibule showed symptomimprovement in all 10 cases. While hearing loss followedby air bubble in the cochlea(5 cases) did not showsymptom improvement. No correlation was found betweenSymptom improvement and otic capsule involvement.Conclusion: In cases of traumatic pneumolabyrinth there area few factors that can be considered to predict the prognosis.Location of the air bubble appears to be the key factor to pre-dicting the prognosis.doi:10.1017/S0022215116005053ID: IP009Sem Study on Cholesteatoma-affectedOssiclesPresenting Author: Maurizio BarbaraMaurizio Barbara1, Selenia Miglietta2, Michela Relucenti3,Ezio Battaglione4, Giuseppe Familiari5, Edoardo Covelli61Sapienza University Rome, 2Department ofAnatomy, Histology, Forensic Medicine andOrthopaedics, Sapienza University, Rome,Italy, 3Department of Anatomy, Histology,Forensic Medicine and Orthopaedic, SapienzaUniversity, Rome, Italy, 4Department ofAnatomy, Histology, Forensic Medicine andOrthopaedic, Sapienza University, Rome, Italy,5Department of Anatomy, Histology, ForensicMedicine and Orthopedics, SapienzaUniversity, Rome, Italy, 6ENT Unit, NESMOS,Sapienza University, Rome, ItalyLearning Objectives: To shed some light on the erosive pro-cesses that affect the incus in respect to the cholesteatomalocalization.Background: The degree of invasiveness of a cholestea-toma is usually based on some clinical features, such asits extension, relapsing tendency and erosive capacity.For this latter, the ossicular chain, and mostly incus, isusually involved, leading the surgeon to its accuratecleansing or removal.Material and Methods: For this study, incus specimens werecollected during tympanomastoid surgery from adult patientswith cholesteatomatous otitis media. The samples were pro-cessed for scanning electron microscopy and the investiga-tion aimed to consider at first the whole bone, then to givea detailed mapping of the eroded parts of the incus adjacentto the cholesteatoma tissue. The different degrees of erosion(in terms of presence/absence of erosion, lacunae and theirdiameter and depth) and the presence/absence of biofilmwere considered. Erosion degree was recorded with 0 ifabsent, and with 1–2–3 if mild, moderate or severe, respect-ively. Five consecutive fields at 100X magnification, alignedin 3 raws, the first one proximal and the last one distal to thesurgical erosive point were analized. A total of 60 fields foreach raw were observed.Results: Erosion lacunae were clustered on the surface of theeroded areas, their diameter being 75± 15μm. Although aproximal to distal gradient exists, looking to the distributionof the eroded areas, grade 3 erosion was not only limited tothe area proximal to the ossicle erosive edge (first raw) butalso in raw 2 and sometimes scattered up to raw 3. Grade 3erosion was observed around nutrient foramina of the bone(65%).Discussion: Our observations confirm the hypothesis that theerosion degree is higher near the resection edge, but alsoprove that erosion areas of degree 3 can also be observedin regions far from the erosive point.doi:10.1017/S0022215116005065ID: IP010Protocols for Application of Non-EPI DWMRI in CholesteatomaPresenting Author: Maurizio BarbaraMaurizio Barbara1, Alessandro Bozzao2, Edoardo Covelli3,Andrea Romano4, Luigi Volpini3, Veronica Confaloni41Sapienza University Rome, 2Neuroradiology,Sapienza University, Rome, Italy, 3ENT Unit,NESMOS Department, Sapienza University,Rome, Italy, 4Neuroradiology, Sant’AndreaHospital, Rome, ItalyABSTRACTS S153https://doi.org/10.1017/S0022215116005053Downloaded from https:/www.cambridge.org/core. Open University Library, on 04 Feb 2017 at 14:00:07, subject to the Cambridge Core terms of use, available at https:/www.cambridge.org/core/terms.

Sem study on cholesteathoma-affected ossicles

https://www.cambridge.org/core/services/aop-cambridge-core/content/view/B725AB5E3DB1151555629645A7769C2B/S0022215116005053a.pdf/div-class-title-sem-study-on-cholesteatoma-affected-ossicles-div.pdf

SEM STUDY ON CHOLESTEATHOMA-AFFECTED OSSICLES

SEM STUDY ON CHOLESTEATHOMA-AFFECTED OSSICLES

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