Bilirubin oxidase from myrothecium verrucaria physically absorbed on graphite electrodes. Insights into the alternative resting from and the sources of activity loss
The oxygen reduction reaction is one of the most important chemical processes in energy
converting systems and living organisms. Mediator-less, direct electro-catalytic reduction of
oxygen to water was achieved on spectrographite electrodes modified by physical adsorption
of bilirubin oxidases from Myrothecium verrucaria. The existence of an alternative resting
form of the enzyme is validated. The effect on the catalytic cycle of temperature, pH and
the presence of halogens in the buffer was investigated. Previous results on the electrochemistry
of bilirubin oxidase and on the impact of the presence of halogens are reviewed
and reinterpreted
