Increased evidence of relevant HIV-1 epidemic transmission in European countries is being reported, with an increased circulation of non-B-subtypes. Here, we present two recent HIV-1 non-B transmission clusters characterized by NNRTI-related amino-acidic mutations among newly diagnosed HIV-1 infected men, living in Rome (Central-Italy)

Alteri, Claudia

Andreoni, Massimo

Antinori, Andrea

Bertoli, Ada

Ceccherini Silberstein, Francesca

Continenza, Fabio

De Carli, Gabriella

Fabeni, Lavinia

Forbici, Federica

Girardi, Enrico

Giuliani, Massimo

Gori, Caterina

Mastroianni, Claudio Maria

Montella, Francesco

Nicastri, Emanuele

Orchi, Nicoletta

Pennica, Alfredo

Perno, Carlo Federico

Pinnetti, Carmela

Santoro, Maria Mercedes

English

PubMed

<div>BackgroundIncreased evidence of relevant HIV-1 epidemic transmission in European countries is being reported, with an increased circulation of non-B-subtypes. Here, we present two recent HIV-1 non-B transmission clusters characterized by NNRTI-related amino-acidic mutations among newly diagnosed HIV-1 infected men, living in Rome (Central-Italy).MethodsPol and V3 sequences were available at the time of diagnosis for all individuals. Maximum-Likelihood and Bayesian phylogenetic-trees with bootstrap and Bayesian-probability supports defined transmission-clusters. HIV-1 drug-resistance and V3-tropism were also evaluated.ResultsAmong 534 new HIV-1 non-B cases, diagnosed from 2011 to 2014, in Central-Italy, 35 carried virus gathering in two distinct clusters, including 27 HIV-1 C and 8 CRF17_BF subtypes, respectively. Both clusters were centralized in Rome, and their origin was estimated to have been after 2007. All individuals within both clusters were males and 37.1% of them had been recently-infected. While C-cluster was entirely composed by Italian men-who-have-sex-with-men, with a median-age of 34 years (IQR:30–39), individuals in CRF17_BF-cluster were older, with a median-age of 51 years (IQR:48–59) and almost all reported sexual-contacts with men and women. All carried R5-tropic viruses, with evidence of atypical or resistance amino-acidic mutations related to NNRTI-drugs (K103Q in C-cluster, and K101E+E138K in CRF17_BF-cluster).ConclusionsThese two epidemiological clusters provided evidence of a strong and recent circulation of C and CRF17_BF strains in central Italy, characterized by NNRTI-related mutations among men engaging in high-risk behaviours. These findings underline the role of molecular epidemiology in identifying groups at increased risk of HIV-1 transmission, and in enhancing additional prevention efforts.</div

Lavinia Fabeni (103620)

Claudia Alteri (288019)

Nicoletta Orchi (782897)

Caterina Gori (103641)

Ada Bertoli (152483)

Federica Forbici (103645)

Francesco Montella (782898)

Alfredo Pennica (782899)

Gabriella De Carli (782900)

Massimo Giuliani (208267)

Fabio Continenza (782901)

Carmela Pinnetti (782902)

Emanuele Nicastri (310235)

Francesca Ceccherini-Silberstein (103660)

Claudio Maria Mastroianni (373442)

Enrico Girardi (78640)

Massimo Andreoni (103649)

Andrea Antinori (103654)

Maria Mercedes Santoro (103615)

Carlo Federico Perno (103671)

The Francis Crick Institute

Recent Transmission Clustering of HIV-1 C and CRF17_BF Strains Characterized by NNRTI-Related Mutations among Newly Diagnosed Men in Central Italy

Lavinia Fabeni

Claudia Alteri

Nicoletta Orchi

Caterina Gori

Ada Bertoli

Federica Forbici

Francesco Montella

Alfredo Pennica

Gabriella De Carli

Massimo Giuliani

Fabio Continenza

Carmela Pinnetti

Emanuele Nicastri

Francesca Ceccherini-Silberstein

Claudio Maria Mastroianni

Enrico Girardi

Massimo Andreoni

Andrea Antinori

Maria Mercedes Santoro

Carlo Federico Perno

Crossref

PLoS ONE

Increased evidence of relevant HIV-1 epidemic transmission in European countries is being reported, with an increased circulation of non-B-subtypes. Here, we present two recent HIV-1 non-B transmission clusters characterized by NNRTI-related amino-acidic mutations among newly diagnosed HIV-1 infected men, living in Rome (Central-Italy).Pol and V3 sequences were available at the time of diagnosis for all individuals. Maximum-Likelihood and Bayesian phylogenetic-trees with bootstrap and Bayesian-probability supports defined transmission-clusters. HIV-1 drug-resistance and V3-tropism were also evaluated.Among 534 new HIV-1 non-B cases, diagnosed from 2011 to 2014, in Central-Italy, 35 carried virus gathering in two distinct clusters, including 27 HIV-1 C and 8 CRF17_BF subtypes, respectively. Both clusters were centralized in Rome, and their origin was estimated to have been after 2007. All individuals within both clusters were males and 37.1% of them had been recently-infected. While C-cluster was entirely composed by Italian men-who-have-sex-with-men, with a median-age of 34 years (IQR:30-39), individuals in CRF17_BF-cluster were older, with a median-age of 51 years (IQR:48-59) and almost all reported sexual-contacts with men and women. All carried R5-tropic viruses, with evidence of atypical or resistance amino-acidic mutations related to NNRTI-drugs (K103Q in C-cluster, and K101E+E138K in CRF17_BF-cluster).These two epidemiological clusters provided evidence of a strong and recent circulation of C and CRF17_BF strains in central Italy, characterized by NNRTI-related mutations among men engaging in high-risk behaviours. These findings underline the role of molecular epidemiology in identifying groups at increased risk of HIV-1 transmission, and in enhancing additional prevention efforts

Directory of Open Access Journals

Recent Transmission Clustering of HIV-1 C and CRF17_BF Strains Characterized by NNRTI-Related Mutations among Newly Diagnosed Men in Central Italy.

Maximum likelihood (ML) tree of the 75 C sequences plus the 4 C references (A). The ML tree was inferred by using PhyML program. Transmission clusters were identified by a bootstrap support >90% (clusters defined by the grey box). The tree was rooted using a midpoint rooting. Bayesian phylogenetic tree of the 75 C sequences plus the 4 C references (B). The Bayesian phylogenetic tree was inferred by using MrBayes. Clades with a posterior probability of one were considered epidemiological clusters (defined by the grey box).</p

Recent Transmission Clustering of HIV-1 C and CRF17_BF Strains Characterized by NNRTI-Related Mutations among Newly Diagnosed Men in Central Italy - Fig 1

PENNICA, Alfredo

Archivio della ricerca- Università di Roma La Sapienza

Recent transmission clustering of HIV-1 C and CRF17_BF strains characterized by NNRTI-related mutations among newly diagnosed men in central Italy

https://figshare.com/articles/_Recent_Transmission_Clustering_of_HIV_1_C_and_CRF17_BF_Strains_Characterized_by_NNRTI_Related_Mutations_among_Newly_Diagnosed_Men_in_Central_Italy_/1509988

Recent transmission clustering of HIV-1 C and CRF17_BF strains characterized by NNRTI-related mutations among newly diagnosed men in central Italy

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The Francis Crick Institute

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Directory of Open Access Journals

The Francis Crick Institute

Archivio della ricerca- Università di Roma La Sapienza