This study reports Madurella tropicana and Madurella pseudomycetomatis as pathogens causing eumycetoma in Senegal. The dominant species, Madurella mycetomatis, shares morphological features with both. Accurate species-level identification is crucial for treatment and epidemiology. Molecular diagnostic tools, especially ITS gene sequencing, enabled the identification of these species. Itraconazole treatment showed a favorable outcome in a patient infected with Madurella pseudomycetomatis, leading to sinus closure.</p
