Založba Univerze v Ljubljani / University of Ljubljana Press
Doi
Abstract
Slovenian autochthonous Cika and Croatian autochthonous Busha cattle were characterized by using nine microsatellites. For each locus, allelic frequencies and heterozygosity were computed. The number of alleles identified for studied polymorphic loci varied from six to ten in Cika population and from four to eight in Busha population. The mean number of alleles per locus was eight (Cika) and five (Busha), respectively. The average expected heterozygosity (Hexp) for the nine loci ranged between 0.7733 (Cika) and 0.7199 (Busha). The highest Hexp was computed for locus BM2113 (0.8521) in Cika and 0.8099 in Busha, suggesting that this locus could be the most informative for both populations. Observed heterozygosity ranged from 0.6316 to 0.825 in Cika and from 0.6667 to 1.0 in Busha indicating a high level of polymorphism for this set of markers. The average observed heterozygosity was slightly lower in Cika (0.7403) compared to Busha population (0.7775). Both breeds were in genetic equilibrium according to the Hardy- Weinberg test. Nei’s standard genetic distance D (Nei, 1978) was 0.065. The genetic difference between the two breeds could be in accordance with their brachycerous origin. The used set of markers proved informative and robust for genotyping in both Cika and Busha breeds. This set of markers can now be applied for screening higher numbers of animals to analyze possible subpopulation structures, improve our knowledge of origin and phylogenetic relationships to other breeds and help to construct efficient preservation plans for these two endangered breeds.Karakterizirali smo slovensko avtohtono pasmo ciko in hrvaško avtohtono pasmo bušo z uporabo devetih mikrosatelitov. Za vsak lokus smo izračunali frekvence alelov in heterozigotnost. Določili smo šest do deset alelov na polimorfnih lokusih v populaciji cike in štiri do osem v populaciji buše. Povprečno število alel na lokus je bilo osem (cika) oziroma pet (buša). Povprečna pričakovana heterozigotnost za devet lokusov je bila med 0,7733 (cika) in 0,7199 (buša). Najvišjo pričakovano heterozigotnost smo izračunali za lokus BM2113, in sicer 0,8521 pri ciki in 0,8099 pri buši. Ta lokus bi tako lahko bil najbolj informativen pri analiziranju obeh populacij. Povprečna opazovana heterozigotnost je bila med 0,6316 in 0,825 pri ciki ozirmoma med 0,6667 in 1,0 pri buši, kar potrjuje visoko informativnost izbranega niza genetskih označevalcev. Pri obeh pasmah smo opazili visoko stopnjo polimorfnosti na vseh devetih lokusih. Povprečna opažena heterozigotnost je bila manjša (0,7403) pri ciki kot pri buši (0,7775). Obe pasmi sta bili v genetskem ravnotežju v skladu s Hardy-Weinbergovim testom. Standardna genetska distanca po Neiju (Nei, 1978) je znašala 0,065, kar je v skladu z njunim brahicernim izvorom. Izbrani niz označevalcev je torej informativen in robusten za genotipiziranje obeh pasem. Ta niz bi v prihodnje lahko uporabili za podrobnejše analize genetskih odnosov do drugih pasem, iskanje možnih pod-populacij znotraj teh pasem ter za razvoj učinkovitega načrta ohranjanja obeh ogroženih pasem
