P-31 and H-1 studies of ethanolamine-linked phosphoglycerides metabolism in human T Lymphocites

Abstract

Abstract: Aqueous and organic extracts of peripheral human T Iymphocytes and of T lymphoblastoid cell lines have been examinated by P-31 and H-1 NMR spectroscopy in order to study the metabolism of ethanolamine (Etn) linked phosphoglycerides. The results show that the Etn concentration in the culture medium determines the composition of Etn-containing metabolites and phospholipids. The effect of phorbol esters, stimulating the synthesis and the breakdown of choline-containing phospholipids has been also studied. A phorbol 12-myristate 13-acetate (PMA) dependent membrane phosphatidylethanolamine hydrolysis, presumably mediated by protein kinase C activity, has been demonstrated

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