Indonesia is a country with abundant agricultural biological resources. One of the plants as a biological source is sago. Sago processing wastes such as bark and waste about 72%. Jepara district has rich sago waste, piled on the side of the road and the river so it is very disturbing. In generally, sago industrial wastes utilization is still lacking, especially as a source of energy. Sago waste consists mainly of cellulose and has the potential to be processed into bioethanol. Glucose contained in cellulosic biomass is the main ingredient in the manufacture of bioethanol and need to know the glucose content after of sago waste cellulose hydrolysis process to determine the highest amount of ethanol. This study aims to determine the glucose content of sago wastewater using acid catalysis with different concentrations of the hydrolysis process, and to know the appropriate concentration of acid to produce the highest glucose and bioethanol in all type of waste. The result showed that type of waste had no effect on glucose content. Glucose content of sago waste showed no difference between the effect of chlorida acid concentration with glucose content. However, hydrolysis at concentration tends to produce the highest glucose
