Bertoldi, Carlo

Consolo, Ugo

Generali, Luigi

Lucchi, A.

Martani, M.

Zaffe, Davide

BERTOLDI, Carlo

GENERALI, Luigi

ZAFFE, Davide

CONSOLO, Ugo

Archivio istituzionale della ricerca - Università di Modena e Reggio Emilia

18 September 2017intestazione repositorydell’ateneoThe influence of composite resin restoration on gingival tissue: a pilot study / Bertoldi, C.; Martani, M.; Generali, L.;Lucchi, A.; Zaffe, D.; Consolo, U.. - In: MINERVA STOMATOLOGICA. - ISSN 0026-4970. - ELETTRONICO. - 64(2015),pp. 151-151. ((Intervento presentato al convegno Prevenzione globale, salute orale e alimentazione tenutosi a Milano nel9,10,11 aprile 2015.OriginalThe influence of composite resin restoration on gingival tissue: a pilot studyPublisher:PublishedDOI:Terms of use:openAccessPublisher copyright(Article begins on next page)Testo definito dall’ateneo relativo alle clausole di concessione d’usoAvailability:This version is available at: 11380/1066256 since: 2015-04-15T12:51:47ZCollegio dei Docenti di OdontoiatriaThis is the peer reviewd version of the followng article:V O L U M E  6 4  .  S U P P L. 1  .  N. 2  .   A P R I L  2 0 1 5XXII Congresso NazionaleCollegio dei Docenti Universitaridi Discipline OdontostomatologicheMilano, 9-11 aprile 2015This journal is PEER REVIEWED and is quoted in: CAB, EMBASE, Index to Dental Literature, PubMed/MEDLINE, ScopusDirezione, redazione, ufficio grafico, ufficio pubblicità, fotocomposizione, amministrazione - Edizioni Minerva Medica - Corso Bramante 83-85 - 10126 TorinoTel. (011) 67.82.82 - Fax (011) 67.45.02 - E-mail: minervamedica@minervamedica.itWeb Site: www.minervamedica.itStampa - Edizioni Minerva Medica - Tipografia di Saluzzo - Corso IV Novembre 29-31 - 12037 Saluzzo (CN) - Tel. 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Autorizzazione del Tribunale di Torino n. 688 del 18-1-1952. Iscrizione nel registro nazionale della stampa di cui allalegge 5-8-1981 n. 416 art. 11 con il numero 00 148 vol. 2 foglio 377 in data 18-8-1982. Pubblicazione periodica mensile - Poste Italiane S.p.A. - Sped. in a.p. - D. L. 353/2003 (conv. in L. 27/02/2004 N° 46) art. 1, comma 1, DCB/CNSegreteria - Società Italiana di Odontostomatologia e Chirurgia Maxillo-Facciale c/o Università Cattolica del Sacro Cuore, Complesso Integrato Columbus, Via G. Moscati 31-33, 00100 Roma. Tel. 06 3503874 - E-mail: siocmf@hotmail.itSegretario e Tesoriere Prof. M. Procaccini.MINERVA STOMATOLOGICA REDATTORE CAPO  DIRETTORE SCIENTIFICO C. Paganelli P. L. 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OLIAROcontinuazione di: MINERVA ORTOGNATODONTICAORGANO UFFICIALE DELLA SOCIETÀ ITALIANADI ODONTOSTOMATOLOGIA E CHIRURGIA MAXILLO-FACCIALEDIRETTORE RESPONSABILEA. OliaroCONSIGLIO DIRETTIVO S.I.O.C.M.F. Presidente Vice Presidenti Segretario generale Segretario Tesoriere D. Cicciù C. Maggiore, C. Paganelli E. Rapisarda M. ProcacciniConsiglieriM. Bosco, S. Caputi, A. Carrassi, U. Covani, C. De Bernardi,G. P. Farronato, E. Gherlone, C. Mortellaro, A. Polimeni, F. ZaroneRevisori dei ContiA. Forabosco, G. Gallina, S. PizziPast President - Probi ViriE. Bertelli, R. Grandini, G. Nidoli, S. Pelo, P. L. SapelliPubblicazione associata a La Rivista aderisce al Codice di Autodisciplina degli Editori Medico Scientificiassociati a FARMAMEDIA e può essere oggetto di pianificazione pubblicitariaVol. 60 - Suppl. 1 al N. 3 MINERVA GASTROENTEROLOGICA E DIETOLOGICA IIIMINERVA ORTOP TRAUMATOL 2014;65(Suppl. 1 al N. 3):XXII Congresso NazionaleCollegio dei Docenti Universitaridi Discipline OdontostomatologicheMilano, 9-11 aprile 2015Anno: 2013Mese: DicembreVolume: 64No: 0Rivista: MINERVA ORTOPEDICA E TRAUMATOLOGICACod Rivista: MINERVA ORTOP TRAUMATOLLavoro: 3060-MOTtitolo breve: xxxxprimo autore: yyyypagine: Vol. 64 - Suppl. 1 al No. 2 MINERVA STOMATOLOGICA 151Aptamers improve cell biocompatibility of implantable biomaterials L. Parisi 1, M. Piergianni 2, A. Smerieri 1, G. Graiani 1, S. Lumet-ti 2, E. Manfredi 1, G. Macaluso 2, M. Bonanini 2, C. Galli 2 1Centro universitario di Odontoiatria, Parma2Dip. Sc. Biomediche, Biotecnologiche e Traslazionali, ParmaAim. Biomaterials, once inserted into a tissue, ad-sorb plasma proteins, which trigger and control the subsequent host reactions. To stimulate undifferenti-ated precursors to commit to the desired lineage, im-plantable biomaterials have been coated with different bioactive molecules, which can enhance tissue regen-eration. A possible approach is to coat biomaterials with elements able to dock a specific peptide, such as aptamers. Aptamers are oligonucleotides able to bind and sequester specific proteins on the surface of the scaffold, enriching it for desired protein spe-cies, to control and direct tissue responses. The aim of the present study was to investigate whether im-mobilised anti-Fibronectin aptamers could selectively enrich hydrogel scaffolds for Fibronectin and promote the attachment and growth of osteoblastic cells. This preliminary study aimed to serve as a proof of concept for a novel class of biomimetic coatings, which could selectively sequester useful proteins on biomaterial surfaces. Methods. We had anti-human Fibronectin DNA aptamers screened for and functionalized with a thiol group on their 3’ end. Polyethyleneglycole diacrylate/thiolated Hyaluronic Acid hydrogels (PEGDA/tHA) were selected as 3D matrix. Aptamers were first im-mobilised on hydrogels and incubated with DMEM enriched with 10% human serum (HS). Fibronectin binding on hydrogels was investigated by spectro-photometry and immunofluorescence. To investigate aptamer effect on cell adhesion, we plated primary human osteoblasts (hOB) on the surface of PEGDA/tHA hydrogels in the presence or in the absence of aptamers and added DMEM enriched with 10% HS in 24 well plates. Cells were cultured on hydrogels for 10 days, rinsed with PBS and fixed for microscopy. Cells were also stained for fluorescent labelling of fo-cal adhesions, microfilaments and nuclei. We quan-titated cell number on hydrogels by MTT assay. We then encapsulated hOBs in PEGDA-tHA hydrogels in the presence or in the absence of aptamers in DMEM enriched with 10% HS. After 10 days, gels were fixed, paraffin enclosed and cut. Sections were then stained with hematoxylin-eosin and analyzed at transmission microscope. Hydrogels were then implanted in sub-cute pouches in rats and harvested 4 and 7 days after surgery for histology. Results. Aptamers specifically bound to Fibronec-tin and enriched hydrogel scaffolds for this protein. The influence of composite resin restoration on gingival tissue: a pilot study C. Bertoldi 1, M. Martani 1, L. Generali 1, A. Lucchi 1, D. Zaffe 2, U. Consolo 1 1Department of Surgery, Medicine, Dentistry and Morpho-logical Sciences with Transplant Surgery, Oncology and Re-generative Medicine Relevance, University of Modena and Reggio Emilia, Modena, Italy2Department of Biomedical, Metabolic And Neural. Scienc-es, Modena, ItalyAim. The study is aimed to compare human gingival tissue close to composite cement restorations with the physiologic situation to natural dental hard tissue at the dental neck of the same tooth. Methods. Eight healthy patients with almost a tooth suprabony jeopardized at the neck/root zone requir-ing endodontic-conservative therapy, were treated by composite build-up (Esthet.X®) to ensure marginal tis-sue stability and to allow secure dental matrix appo-sition. Esthet.X® was used to treat the defect but the perimeter of the residual coronal tooth had to present at least a subgingival restored section and another section consisting of natural enamel to permit subsequent com-parison of the gingival tissues close to the natural hard tissues of the tooth (control site) and to the composite restorations (test site). Three months after the Esthet.X® build-up, the crown lengthening surgical procedure was performed to restore the physiological dimension of the biological space. During the surgical procedure, the secondary flap was harvested, fastened to a semi-rigid support, and histologically examined using the following grade scale of inflammation: 1) Absence or occasional (0-3), 2) Weak (4-19), 3) Moderate (20-99), 4) High (100-499) and 5) Severe (500 and above). The statistical analysis was performed by the non-parametric Mann-Whitney test. Results. The histological analysis showed an uneven distribution of inflammatory cells in the corion of gingi-va portions, both close to the Esthet.X® restorations and the dental hard tissues, in all biopsies. The inflammation grade varied from severe to weak. The statistical analysis did not show any significant difference between the co-rion of gingival tissue close to the restoration and dental hard tissue surface. Conclusion. No information exists about the behav-ior of human gingival tissue close to composite cements used to treat crown and dental neck defects. The reac-tion of the human gingival to composite build up per-formed in the dental neck area and the coronal part of the dental root was analyzed in this study and within the limitations of this pilot study, the results seem to indicate that use of this kind of composite does not so much alter greatly the gingival tissue. Anno: 2015Mese: AprilVolume: 64No: 2Rivista: MINERVA STOMATOLOGICACod Rivista: MINERVA STOMATOLLavoro: 2161-MGASTRtitolo breve: primo autore: pagine: 1-2PARODONTOLOGIA

The influence of composite resin restoration on gingival tissue: a pilot study

The influence of composite resin restoration on gingival tissue: a pilot study

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