The genotypic false positive rate determined by V3 population sequencing can predict the burden of HIV-1 CXCR4-using species detected by pyrosequencing

Abbate, I; Andreoni, M; Antinori, A; Armenia, D; Bruselles, A; Capobianchi, M; Ceccherini Silberstein, F; Cento, V; Fabeni, L; Forbici, F; Gori, C; Ippolito, G; Latini, A; Micheli, V; Palamara, G; Perno, CF; Rizzardini, G; Rozera, G; Santoro, M; Svicher, V

research

The genotypic false positive rate determined by V3 population sequencing can predict the burden of HIV-1 CXCR4-using species detected by pyrosequencing

Authors: I Abbate
M Andreoni
A Antinori
D Armenia
A Bruselles
M Capobianchi
F Ceccherini Silberstein
V Cento
L Fabeni
F Forbici
C Gori
G Ippolito
A Latini
V Micheli
G Palamara
CF Perno
G Rizzardini
G Rozera
M Santoro
V Svicher
Publication date: 1 January 2013
Publisher: Public Library of Science
Doi

Abstract

The false-positive rate (FPR) is a percentage-score provided by Geno2Pheno-algorithm indicating the likelihood that a V3-sequence is falsely predicted as CXCR4-using. We evaluated the correlation between FPR obtained by V3 population-sequencing and the burden of CXCR4-using variants detected by V3 ultra-deep sequencing (UDPS) and Enhanced-Sensitivity Trofile assay (ESTA)