Anticoagulant polymer sulfated hyaluronic acid was patterned immobilized on a poly(ethylene
terephthalate) (PET) film in a specific pattern by photolithography. Hyaluronic acid was sulfated by
a sulfur trioxide-pyridine complex. The polymer was coupled with azidoaniline. The derivatized
polymer was cast on a PET film from aqueous solution. After drying, the film was photoirradiated in
the presence or absence of a photomask. The micropatterning was confirmed by staining with a dye,
brilliant green. Since the anticoagulant polymer has negative charges, the cationic dye was adsorbed
on the regions where the anticoagulant polymer was immobilized. Platelet adhesion was reduced on
the sulfated hyaluronic acid-immobilized areas. The immobilized sulfated hyaluronic acid significantly
reduced thrombus formation
