BUGNOLI M

DE BERNARD M

MONTECUCCO C

N. FIGURA

PAPINI E

RAPPUOLI R

Bafilomycin A1, a specific inhibitor of the vacuolar‐type H+‐ATPase, responsible for acidification of intra‐cellular compartments, prevents the vacuolization of Hela cells induced by H. pylori, with an inhibitory concentration giving 50% of maximal (ID50) of 4 nM. Bafilomycin A1 is also very efficient in restoring vacuolated cells to a normal appearance. The vacuolating activity of Helicobacter pylori is not inhibited by a series of specific inhibitors of vacuolar H+‐ATPases. These findings indicate that a transmembrane pH gradient is needed for the formation and growth of vacuoles caused by the bacterium and that this pH gradient is due to the activity of a vacuolar ATPase proton pump of HeLa cells. Copyright © 1993, Wiley Blackwell. All rights reserved

Papini, E.

Bugnoli, M.

De Bernard, M.

Figura, N.

Rappuoli, R.

Montecucco, C.

Archivio della Ricerca - Università degli Studi di Siena

Bafilomycin  A1 inhibits Helicobacter  pylori-induced  vacuolization on HeLa  cells

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Bafilomycin  A1   inhibits Helicobacter  pylori-induced  vacuolization on  HeLa  cells

Bafilomycin A1 inhibits Helicobacter pylori-induced vacuolization on HeLa cells

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Archivio della Ricerca - Università degli Studi di Siena

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