open7openNICOLAI, Piero; MENSI, Magda; Marsili, F.; Piccioni, M.; SALGARELLO, Stefano Alessandro; GILBERTI, Maria Enrica; Apostoli, P.Nicolai, Piero; Mensi, Magda; Marsili, F.; Piccioni, M.; Salgarello, Stefano Alessandro; Gilberti, Maria Enrica; Apostoli, P

Apostoli, P.

Gilberti, E.

Marsili, F.

Mensi, M.

Nicolai, P.

Piccioni, M.

Salgarello, S.

NICOLAI, Piero

MENSI, MAGDA

SALGARELLO, Stefano Alessandro

GILBERTI, Maria Enrica

Archivio istituzionale della ricerca - Università di Padova

93ACTA oTorhinolAryngologiCA iTAliCA 2015;35:93-96RhinologyMaxillary fungus ball: zinc-oxide endodontic materials as a risk factorIl Fungus Ball Mascellare: il materiale endodontico a base di ossido di zinco  come fattore di rischioP. Nicolai1, M. MeNsi2, F. Marsili2, M. PiccioNi1, s. salgarello2, e. gilberti3, P. aPostoli31 Department of otorhinolaryngology, University of brescia, italy; 2 Department of endodontology, Dental clinic, University of brescia, italy; 3 Department of occupational Medicine, University of brescia, italySummAryThe objective of this study was to demonstrate the correlation between endodontic treatment on maxillary teeth and fungus ball with inductively coupled plasma mass spectrometry measurement of zinc and other metals (barium, lead and copper) in fungus ball samples. Samples of normal maxillary mucosa were used as comparison. metal concentration was also measured in several endodontic materials. A significant difference was found between the concentration of zinc and copper in fungus ball compared to normal mucosa. metal distribu-tion was more similar in fungus ball and in the endodontic materials tested than normal mucosa. The similar metal concentration in the endodontic materials and fungus ball suggests that endodontic materials play a role in the pathogenesis of fungus ball. Endodontic materials accidentally pushed into the maxillary sinus during endodontic treatments may play a crucial role. Dentists should be as careful as possible when treating maxillary teeth to avoid perforating the maxillary sinus floor; the use of zinc-free endodontic materials, as zinc is a metal that plays a pivotal role in fungus growth, should be encouraged.KEy worDS: Maxillary fungus ball • Zinc-oxide materials • Endodontic treatment • Inductively coupled plasma mass spectrometryriASSunToL’obiettivo di questo studio è stato dimostrare la correlazione tra il trattamento endodontico su denti mascellari antrali e il Fungus Ball mascellare. mediante L’utilizzo della ICP-MS (Inductively Coupled Plasma Mass Spectrometry) è stata utilizzata per la misurazione della concentrazione di zinco e altri metalli (bario, piombo e rame) in campioni di Fungus Ball. Per il confronto sono stati utilizzati campioni di mucosa sana del seno mascellare. La concentrazione di questi degli stessi metalli è stata misurata anche in diversi materiali utilizzati per le terapie endodontiche. Vi era è una differenza significativa tra la concentrazione di zinco e rame nei campioni di Fungs Ball rispetto alla mucosa sana. La concentrazione del dei metallo metalli nei fungus ball e nei materiali endodontici testati è invece sovrapponibile è stata sovrapponibile tra il Fungus Ball e i materiali endodontici testati. Tale dato La concentrazione simile nei materiali endodontici e nel Fungus Ball suggerisce che il materiale endodontico svolga un ruolo nella patogenesi del Fungus Ball. Ciò suggerisce che i materiali endodontici inavvertitamente spinti nel seno mascellare durante i trattamenti canalari possano svolgere un ruolo fondamentale. I dentisti dovrebbero essere il più attenti possibile durante il trattamento di denti dell’arcata superiore per evitare di forare il pavimento del seno mascellare. e forse L’uso di materiali endodontici privi di zinco dovrebbe essere incoraggiato, essendo lo zinco un metallo che gioca un ruolo fondamentale nella crescita del fungo.PArolE ChiAvE: Fungus ball mascellare • Materiali a base di ossido di zinco • Trattamento endodontico • ICP-MS (Inductively Coupled Plasma Mass Spectrometry)Acta Otorhinolaryngol Ital 2015;35:93-96IntroductionContinuity or contiguity of the upper teeth apical dental root foramina (molars and premolars, more rarely ca-nines) with the maxillary sinus floor 1-3 may cause spread of odontogenic inflammatory and/or infective processes to the maxillary sinus. The most frequent infective pro-cess is the maxillary sinusitis and a less frequent form of possible odontogenic origin is a non-invasive my-cotic form: fungus ball (FB)  4-7  5-8. The most credible aetiology of FB is endodontic treatment of the upper teeth  2  8-12  2  4  9-12. The time interval between endodontic therapy and diagnosis of FB is several years (6 on aver-age) 11. usually, symptoms appear when the fungal mass fills the entire maxillary sinus. At this point, surgery be-comes the only viable option.it is well-known that endodontic sealers, long used in den-tal practice, are mostly made of zinc oxide, and it has been demonstrated 13 14 that zinc promotes fungal growth as it is an essential microelement for helping some fungi such as P. Nicolai et al.94Aspergillus sp. (which is the main pathogen leading to FB) survive and proliferate 5 7 6 8.in a previous paper  13, we showed that eugenol and the mixture of powder-eugenol of endodontic sealers inhibit the growth of Aspergillus. Even after 75 days, eugenol has a slight inhibitory effect on such growth. The amount of zinc oxide in powders has a direct bearing on their effec-tiveness in promoting Aspergillus growth. Therefore, it is likely that endodontic sealer may cause the development of mycotic sinusitis as eugenol gradually loses its ability to inhibit the growth of Aspergillus, leaving zinc oxide, which is essential for the growth of Aspergillus.over a series of 102 patents with FB, clinical and radio-logic diagnosis showed the presence of endodontically-treated teeth or post-extraction sites of previously endo-dontically-treated teeth in 99% of cases 9.This is, however, only a clinical observation. Thus, is it suf-ficient to justify the odontogenic origin of FB? The objective of this prospective study was to evaluate the presence of zinc and other metals contained in the most important endodontic sealers in FB samples and in the adjacent mucosa in order to determine if there is a statistically significant difference.Materials and methodsFrom october 2002 to September 2007, 53 patients affect-ed by FB were surgically treated by endoscopic surgery at the Department of otorhinolaryngology, university of Brescia (italy). All patients were also examined in the Dental Clinic before undergoing surgical treatment. De-mographics and historical data were collected along with radiographic findings (panoramic, intraoral periapical ra-diographs and CT of the sinuses).The treatment consisted in the complete removal of the fungal debris with reaeration of the sinus through an en-doscopic minimally-invasive approach  15  31. During the surgery a sample of normal mucosa was taken from the maxillary sinus.After the surgical procedure, FB and maxillary mucosa samples were sent to the Pathology Department to con-firm diagnosis and to the industrial Toxicology laboratory of the Section of occupational medicine and industrial hygiene of the Department of Experimental and Applied medicine to perform inductively coupled plasma mass spectrometry (iCP-mS).using the same method, the following endodontic mate-rials were also examined in the occupational medicine laboratory using the iCP-mS method 16 20:PCS-Pulp Canal Sealer (Kerr, romulus - mi 48174, uSA);n2-Sargenti Cement (hager & werken gmbh & Co Kg, Duisburg 47006, germany);Endometasone (Septodont, Saint-maur-Des-Fossès, Ce-dex 94107, France)Standardised gutta-percha (Kumapan, Dia Dent group in-ternetional, Choongehong Buk Do. Korea)non-standardised gutta-percha (Dentsply maillefer 1338 Ballaigues Switzerland).Two samples of each material were analysed and the aver-age value of each metal was kept as a reference.moreover, 5 cultures of different fungi (Aspergillus fu-migatus, Aspergillus flavus, Aspergillus niger, Alternaria, bipolaris) were analysed with the same methods.Measuring instruments and proceduresTissue specimens were oven dried at 70°C for 2  h, left to cool at room temperature in a desiccator, and finally weighed. Samples were then dissolved in hno3 (ACS rea-gent, Sigma) 70% (v/v) for 2 h at 70°C.The digested samples (0.1 ml) were diluted with deionised water to 5 ml.Prepared samples underwent iCP-mS analysis on a Perkin Elmer ElAn DrC ii instrument (Perkin Elmer, wood-bridge, on, Canada) using the analytical technique total quant with external calibration.The method’s accuracy was determined in natural wa-ter reference materials (niST 1640, national institute of Standard and Technology, gaithersburg, mD).The coefficients of variation (Cv) ranged from 4 to 8% among series and from 6 to 12% between series. The in-strument was calibrated using a standard solution at a con-centration of 10 µg/l (multielement iCP-mS Calibration Standard 3, matrix per volume: 5% hno3 per 100  ml, Perkin Elmer Plus).The limits of detection (loD) were determined on the ba-sis of three standard deviations (SD) of the background signal, and the following values (as µg/l) were obtained: 0.006 for Bi and Ba; 0.005 for hg; 0.004 for Cu, Sr and Pb; 0.003 for Ag; 0.002 for Zn and Mn 17 30.Statistical analysisThe data are to be considered as average values (Stand-ard Deviation, SD).The non-parametric mann-whitney test was used to compare metal (Zn, Cu) concentrations in the FB and maxillary mucosa. SPSS was used to ana-lyse the data. A p < 0.005 was considered as statistically significant.ResultsAnamnestic and radiographic data showed that 99% of pa-tients had undergone at least one endodontic treatment in the maxillary sector where the FB was found. The results of iCP-mS of elements in endodontic materials are listed in Ta-ble i. Since zinc and copper are widely present in endodontic materials, they may be used as a reliable finger-printing of those materials. in Table ii, the results of FB, mucosa and cultures of fungi are compared to endodontic material.it can be clearly seen that zinc and copper are the elements most widely used in endodontic materials. These metals were considered in statistical analysis to sustain the evi-Fungus ball and endodontic materials95dence for the presence of endodontic materials in FB sam-ples compared to their absence in normal mucosa.various metals may be traced 18 21 in all biological tissues, but this objective of this study was to show a difference between the amounts of zinc and copperin the FB (and therefore of exogenous origin) and that of those to be found in mucosa.FBs were found to contain a quantity of zinc and copper that was more similar to endodontic materials tested than the mucosa samples, while lower average values were de-tected in fungi cultures.The mann-whitney test is significant with p < 0.05 for met-als tested comparing samples of FB and mucosa (Table ii).DiscussioniCP-mS is a highly sensitive, multielement analytical method that has increasingly become affordable to many labs. it is being applied to biological samples and has sev-eral advantages: multielement stimulation determinations, excellent detection limits, a wide linear dynamic range and a high sample throughput 22 18.The results obtained with iCP-mS and statistical analysis show that metals present in the most widely used endodon-tic materials are found in significantly higher amounts in FBs compared to mucosa.Traces of these metals, as found in nasal mucosa, are how-ever compatible with those found in lung tissues and in the upper respiratory tract as a result of atmospheric contamina-tion by microelements such as lead, zinc and barium 20 22<?>ConclusionsFB is a non-invasive mycotic sinusitis. while the incidence of FB is not well known (3.7%) 21 23, it is certainly of sub-stantial relevance as it cannot be treated through medical therapy. Therefore, a surgical procedure is needed 23 24 32 33, which represents an invasive intervention for the patient.The correlation between endodontic therapies and FB as shown in a case control study 9 and supported by different case series  2  5  11  25  2  6  11  26, together with the demonstration, through this study, of the presence of metal contents in en-dodontic material in samples of FB, contributes to strongly support the odontogenic aetiologic hypothesis. moreover, Tung-lung Tsai et al. 26 27 have demonstrated that there is no relation between obstruction of the ostiomeatal complex and FB, thus confirming a different aetiopathogenesis of FB.Despite the fact that research is progressing in this direc-tion, it is crucial that dentists take some simple precautions to prevent this problem from occurring. it is advisable to execute root canal therapies on antral teeth (molars, pre-molars, canines of upper jaw) with the utmost attention to work length (electronic apex locator and intraoral periapi-cal radiographs 27 28) to avoid over-instrumentation, to use warm canal obturation techniques which need low amounts of sealers and possibly endodontic sealers not based on zinc oxide 28 29. The follow-up is present beyond the apex after endodontic treatment should include a radiological check-up and evaluation of possible symptoms at least once a year in order to precociously identify any pathologic signs and to subsequently refer the patient to an EnT specialist.Table I. Average values by ICP-MS of elements in endodontic sealers.Metals in endodontic sealers Metal [µg/g] Cu Zn Pb Ba Mn Ag Bi Sr HgEndometasone 50 395000 17500 22500 3 4,5 35 160 8N2 75 620500 8 0 1.5 160 193000 0 0PCS 70 601000 15 25 7.5 421000 10 8.5 45Non-standardised guttaperca 115 719500 5 2 0 1 0 0.1 0Standardised guttaperca 100 627500 5 1600 7 2.5 0 30 0Cu: copper, Zn: zinc, Pb: lead, Ba: barium, Mn: manganese, Ag: silver, Bi: bismuth, Sr: strontium, Hg: mercury.Table II. Average values and SD of ICP-MS for normal mucosa, fungus ball, endodontic materials and a culture of Aspergillus spores and Mann-Whitney test Results.Metal [µg/g] Cu Zn Endodontic sealers 82.00(25.64)592700(119599)Fungus ball 98.84(128.02)3199(15961.58)Mucosa 15.73(26.09)220.75(965.91)FungI 7.8(5.11)7.37(3.97)Fungus ball vs. mucosa p = 0.000 p = 0.000Cu = copper, Zn = zinc.P. Nicolai et al.96AcknowledgementsThe statistical analysis was made with the collaboration of L.O. Redaelli de Zinis, M.D. Department of Otorhinolaryn-gology university of Brescia, Spedali Civili, Brescia, italy.The manuscript was translated by Dr. Alexander giordano.References1 lenglinger FX, Krennmair g, muller-Schelken h, et  al. Radiodense concrections in maxillary sinus aspergillosis: pathogenesis and the role of CT densitometry. Eur radiol 1996;6:375-9.2 legent F, Billet J, Beauvillain C, et al. The role of dental ca-nal fillings in the development of Aspergillus sinusitis. A re-port of 85 cases. Arch otorhinolaryngol 1989;246:318-20.3 Bertrand B, rombaux P, Eloy P, et al. Sinusitis of dental ori-gin. Acta otorhinolaryngol Belg 1997;51:315-22.4 Beck mannagetta J, neck D. Radiologic finding in aspergil-losis of the maxillary sinus. oral Surg oral med oral Pathol 1986;62:345-9.5 Khongkhunthian P, reichart PA. Aspergillosis of the maxil-lary sinus as a complication of overfilling root canal material into the sinus: report of two cases. J Endod 2001;27:476-8.6 DeShazo rD. Fungal sinusitis. Am J med Sci 1998;316:39-45.7 Stammberger h, Jakse r, Beaufort F. Aspergillosis of the par-anasal sinuses: x-ray diagnosis, histopathology, and clinical aspects. Ann otol laryngol 1984;93:251-6.8 Fligny i, lamas g, rouhani F, et  al. Chronic maxillary si-nusitis of dental origin and nasosinusal aspergillosis. How to manage intrasinusal foreign bodies? Ann otolaryngol Chir Cervicofac 1991;108:465-8.9 mensi m, Piccioni m, marsili F, et al. Risk of maxillary fun-gus ball in patients with endodontic treatment on maxillary teeth: a case-control study. oral Surg oral med oral Pathol oral radiol Endod 2007;103:433-6.10 De Foer C, Fossion E, vaillant Jm. Sinus Aspergillosis. J Cra-niomaxillofac Surg 1990;18:33-40.11 Fancello A, Anghinoni m, Tullio A. Sinusiti mascellari da As-pergillo. minerva Stomatol 1998;47:51-6.12 Beck-mannagetta J, necek D, grasserbauer m. Solitary as-pergillosis of maxillary sinus, a complication of dental treat-ment. The lancet 1983;26:1260.13 mensi m, Salgarello S, Pinsi g, et  al. Mycetoma of the Maxillary sinus: Endodontic and Microbiological Correla-tions. oral Surg oral med oral Pathol oral radiol Endod 2004;98:119-23.14 willinger B, Beck mannagetta J, hirschl Am, et al. Influence of zinc oxide on Aspergillus species: a possible cause of local, non-invasive aspergillosis of the maxillary sinus. mycoses 1996;39:361-6.15 Stammberger h. Endoscopic surgery for mycotic and chronic recurring sinusitis. Ann otol rhinol laringol Suppl 1985;119:1-11.16 Kobayashi A. Asymptomatic aspergillosis of the maxillary si-nus associated with foreign body of endodontic origin. Report of a case. int J oral maxillofac Surg 1995;24:243-4.17 Apostoli P, De Palma g, Catalani S, et  al. Multielemental analysis of tissues from Cangrande della Scala, Prince of Ve-rona, in the 14th century. J Anal Toxicol 2009;33:322-7.18 rodushkin i, odman F, olofsson r, et al. Determination of 60 elements in whole blood by inductively coupled plasma mass spectrometry. J Anal At Spectrom 2000;15:937-44.19 Tanaka h, Sakae T, mishima h, et al. Calcium phosphate in Aspergillosis of the maxillary sinus. Scanning microscopy. 1993;7:1241-6.20 Takemoto K, Kawai h, Kuwahara T, et al. Metal concentra-tions in human lung tissue, with special reference to age, sex, cause of death, emphysema and contamination of lung tissue. int Arch occup Environ health 1991;62:579-86.21 Ferreiro JA, Carlson BA, Cody DT 3rd. Paranasal fungus balls. head neck 1997;19:481-6.22 Zinreich SJ, Kennedy DW, Malat J, et al. Fungal sinusitis: di-agnosis with CT and MR imaging. Head and Neck Radiology 1988;169:439-44.23 Pagella F, matti E, De Bernardi F, et al. Paranasal sinus fungus ball: diagnosis and management. mycoses 2007;50:451-6.24 nicolai P, lombardi D, Tomenzoli D, et  al. Fungus ball of the paranasal sinuses: experience in 160 patients treated with endoscopic surgery. laryngoscope 2009;119:2275-9.25 Theaker ED, rushton vE, Corcoran JP, et al. Chronic sinusi-tis and zinc-containing endodontic obturating pastes. Br Dent J 1995;179:64-8.26 Tsai Tl1, guo yC, ho Cy, et al. The role of ostiomeatal com-plex obstruction in maxillary fungus ball. otolaryngol head neck Surg 2006;134:494-8.27 venturi m, Breschi l. A comparison between two elec-tronic apex locators: an in vivo investigation. int Endod J 2005;38:36-45.28 Peters oA, Barbakov F, Peters Ci. An analysis of endodontic treatment with 3 nickel-titanium rotary root canal prepara-tion techniques. int Endod J 2004;37:849-59.Address for correspondence: Federico marsili, Dental Clinic, uni-versity of Brescia, Spedali Civili, p.le Spedali Civili 1, 25123 Bre-scia, italy. Tel. +39 030 3995784/782/790. Fax +39 030 303194. E-mail: fedemarsili@hotmail.itreceived: December 4, 2013 - Accepted october 2, 2014

English

Maxillary fungus ball: zinc-oxide endodontic materials as a risk factor

Nicolai, Piero

Mensi, Magda

Salgarello, Stefano Alessandro

Gilberti, Maria Enrica

Archivio istituzionale della ricerca - Università di Brescia

Padua Research Archive

MENSI, Magda

https://www.research.unipd.it/bitstream/11577/3311464/1/Pubblicazione%20definitiva%20Acta.pdf

Maxillary fungus ball: zinc-oxide endodontic materials as a risk factor

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Archivio istituzionale della ricerca - Università di Padova

Archivio istituzionale della ricerca - Università di Brescia

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Archivio istituzionale della ricerca - Università di Brescia

Archivio istituzionale della ricerca - Università di Brescia