Mesenchymal stromal cells (MSC) are nonhematopoietic, multipotent progenitor cells that are considered
one of the most promising product for cellular therapy applications.
Initially expanded from bone marrow, MSC can be culture-expanded from different other sources (i.e.,
umbilical cord blood, synovial membrane, dental pulp, placenta). Recently, adipose tissue (AT) has been
regarded as a more suitable and easily accessible source of MSC. The designation of MSC requires
that in vitro-expanded cells be plastic-adherent, express surface CD73, CD90 and CD105 but not the
hematopoietic markers CD14, CD34, CD45 and the ability to differentiate under specific in vitro culture
conditions into osteoblastic, adipocytic and chondroblastic lineages. We will show our characterization
of AT-derived MSC, comparing cell lines derived from subcutaneous (lipoaspirate, L) or visceral
(omental, O) white fat. The results show significant differences between cell lines in terms of growth
characteristics, differentiation properties, immunosuppression capacity, and angiogenic potential of their
conditioned medium. We are convinced that the identification of the peculiarities of MSC isolated from
different tissues will lead to a more targeted use in cell therapy
