Barra, L.

Campbell, K.

Dittami, S.

Edvardsen, B.

Elliott, C.T.

Escalera, L.

Graneli, E.

Hagstrom, J.

Kegel, J.

Kooistra, W.H.C.F.

Lewis, J.

McCoy, G.R.

McNamee, S.E.

Medlin, L.K.

Montresor, M.

Pazos, Y.

Pérez Blanco, E.

Raine, R.

Reguera, B.

Rodríguez, F.

Ruggiero, M.V.

Taylor, J.

English

Harmful algal blooms (HABs) are a natural global phenomena emerging in severity and extent. Incidents have many economic, ecological and human health impacts. Monitoring and providing early warning of toxic HABs are critical for protecting public health. Current monitoring programmes include measuring the number of toxic phytoplankton cells in the water and biotoxin levels in shellfish tissue. As these efforts are demanding and labour intensive, methods which improve the efficiency are essential. This study compares the utilisation of a multitoxin surface plasmon resonance (multitoxin SPR) biosensor with enzyme-linked immunosorbent assay (ELISA) and analytical methods such as high performance liquid chromatography with fluorescence detection (HPLC-FLD) and liquid chromatography–tandem mass spectrometry (LC–MS/MS) for toxic HAB monitoring efforts in Europe. Seawater samples (n = 256) from European waters, collected 2009–2011, were analysed for biotoxins: saxitoxin and analogues, okadaic acid and dinophysistoxins 1/2 (DTX1/DTX2) and domoic acid responsible for paralytic shellfish poisoning (PSP), diarrheic shellfish poisoning (DSP) and amnesic shellfish poisoning (ASP), respectively. Biotoxins were detected mainly in samples from Spain and Ireland. France and Norway appeared to have the lowest number of toxic samples. Both the multitoxin SPR biosensor and the RNA microarray were more sensitive at detecting toxic HABs than standard light microscopy phytoplankton monitoring. Correlations between each of the detection methods were performed with the overall agreement, based on statistical 2 × 2 comparison tables, between each testing platform ranging between 32% and 74% for all three toxin families illustrating that one individual testing method may not be an ideal solution. An efficient early warning monitoring system for the detection of toxic HABs could therefore be achieved by combining both the multitoxin SPR biosensor and RNA microarray

McNamee, Sara

Medlin, L.K. (Linda K.)

Kegel, Jessica

McCoy, G. (Gary)

Raine, R. (Robin)

Barra, Lucia

Ruggiero, Maria-Valeria

Kooistra, Wiebe

Montresor, Marina

Hagström, J.A. (Johannes)

Pérez-Blanco, Eva

Granéli, E (Edna)

Rodríguez, F. (Francisco)

Escalera-Moura, L. (Laura)

Reguera, B. (Beatriz)

Dittami, S.M. (Simon M.)

Edvardsen, B. (Bente)

Taylor, Joe

Lewis, Jane

Pazos, Y. (Yolanda)

Elliot, Christopher

Campbell, Katrina

Repositorio Institucional Digital del IEO

Distribution, occurrence and biotoxin composition of the main

Sara E. McNamee

Linda K. Medlin

Jessica Kegel

Gary R. McCoy

Robin Raine

Lucia Barra

Maria Valeria Ruggiero

Wiebe H.C.F. Kooistra

Marina Montresor

Johannes Hagstrom

Eva Perez Blanco

Edna Graneli

Francisco Rodríguez

Laura Escalera

Beatriz Reguera

Simon Dittami

Bente Edvardsen

Joe Taylor

Jane M. Lewis

Yolanda Pazos

Christopher T. Elliott

Katrina Campbell

Crossref

Harmful Algae

Distribution, occurrence and biotoxin composition of the main shellfish toxin producing microalgae within European waters: A comparison of methods of analysis

Harmful algal blooms (HABs) are a natural global phenomena emerging in severity and extent. Incidents have many economic, ecological and human health impacts. Monitoring and providing early warning of toxic HABs are critical for protecting public health. Current monitoring programmes include measuring the number of toxic phytoplankton cells in the water and biotoxin levels in shellfish tissue. As these efforts are demanding and labour intensive, methods which improve the efficiency are essential. This study compares the utilisation of a multitoxin surface plasmon resonance (multitoxin SPR) biosensor with enzyme-linked immunosorbent assay (ELISA) and analytical methods such as high performance liquid chromatography with fluorescence detection (HPLC-FLD) and liquid chromatography-tandem mass spectrometry (LC-MS/MS) for toxic HAB monitoring efforts in Europe. Seawater samples (n = 256) from European waters, collected 2009-2011, were analysed for biotoxins: saxitoxin and analogues, okadaic acid and dinophysistoxins 1/2 (VDU /DTX2) and domoic acid responsible for paralytic shellfish poisoning (PSP), diarrheic shellfish poisoning (DSP) and amnesic shellfish poisoning (ASP), respectively. Biotoxins were detected mainly in samples from Spain and Ireland. France and Norway appeared to have the lowest number of toxic samples. Both the multitoxin SPR biosensor and the RNA microarray were more sensitive at detecting toxic HABs than standard light microscopy phytoplankton monitoring. Correlations between each of the detection methods were performed with the overall agreement, based on statistical 2 x 2 comparison tables, between each testing platform ranging between 32% and 74% for all three toxin families illustrating that one individual testing method may not be an ideal solution. An efficient early warning monitoring system for the detection of toxic HABs could therefore be achieved by combining both the multitoxin SPR biosensor and RNA microarray. (C) 2016 Elsevier B.V. All rights reserved.</p

McNamee, Sara E.,

Medlin, Linda K.,

Kegel, Jessica,

McCoy, Gary R.,

Raine, Robin,

Barra, Lucia,

Ruggiero, Maria Valeria,

Kooistra, Wiebe H. C. F.,

Montresor, Marina,

Hagström, Johannes,

Pérez Blanco, Eva,

Granéli, Edna,

Rodriguez, Francisco,

Escalera, Laura,

Reguera, Beatriz,

Dittami, Simon,

Edvardsen, Bente,

Taylor, Joe,

Lewis, Jane M.,

Pazos, Yolanda,

Elliott, Christopher T.,

Campbell, Katrina,

Swepub

Distribution, occurrence and biotoxin composition of the main shellfish toxin producing microalgae within European waters : A comparison of methods of analysis

McNamee, SE

Medlin, LK

Kegel, JU

McCoy, GR

Raine, R

Barra, L

Ruggiero, MV

Kooistra, WHCF

Montresor, M

Hagstrom, J

Blanco, EP

Graneli, E

Rodriguez, R

Esclaera, L

Reguera, B

Dittami, S

Edvardsen, B

Taylor, J

Lewis, JM

Pazos, Y

Elliott, CT

Campbell, K

Plymouth Marine Science Electronic Archive (PlyMSEA)

Distribution, occurrence and biotoxin composition of the main shellfish toxin producing microalgae within European waters: A comparison of methods of analysis.

McNamee, Sara E.

Medlin, Linda K.

McCoy, Gary R.

Raine, Robin

Ruggiero, Maria Valeria

Kooistra, Wiebe H.C.F.

Hagstrom, Johannes

Blanco, Eva Perez

Graneli, Edna

Rodríguez, Francisco

Escalera, Laura

Reguera, Beatriz

Dittami, Simon

Edvardsen, Bente

Lewis, Jane M.

Pazos, Yolanda

Elliott, Christopher T.

Access to Research at National University of Ireland, Galway

Distribution, occurrence and biotoxin composition of the main shellfish toxin producing microalgae within european waters: a comparison of methods of analysis

Harmful algal blooms (HABs) are a natural global phenomena emerging in severity and extent. Incidents have many economic, ecological and human health impacts. Monitoring and providing early warning of toxic HABs are critical for protecting public health. Current monitoring programmes include measuring the number of toxic phytoplankton cells in the water and biotoxin levels in shellfish tissue. As these efforts are demanding and labour intensive, methods which improve the efficiency are essential. This study compares the utilisation of a multitoxin surface plasmon resonance (multitoxin SPR) biosensor with enzyme-linked immunosorbent assay (ELISA) and analytical methods such as high performance liquid chromatography with fluorescence detection (HPLC-FLD) and liquid chromatography–tandem mass spectrometry (LC–MS/MS) for toxic HAB monitoring efforts in Europe. Seawater samples (n = 256) from European waters, collected 2009–2011, were analysed for biotoxins: saxitoxin and analogues, okadaic acid and dinophysistoxins 1/2 (DTX1/DTX2) and domoic acid responsible for paralytic shellfish poisoning (PSP), diarrheic shellfish poisoning (DSP) and amnesic shellfish poisoning (ASP), respectively. Biotoxins were detected mainly in samples from Spain and Ireland. France and Norway appeared to have the lowest number of toxic samples. Both the multitoxin SPR biosensor and the RNA microarray were more sensitive at detecting toxic HABs than standard light microscopy phytoplankton monitoring. Correlations between each of the detection methods were performed with the overall agreement, based on statistical 2   2 comparison tables, between each testing platform ranging between 32% and 74% for all three toxin families illustrating that one individual testing method may not be an ideal solution. An efficient early warning monitoring system for the detection of toxic HABs could therefore be achieved by combining both the multitoxin SPR biosensor and RNA microarray

WestminsterResearch

University of Galway Research Repository

Harmful algal blooms (HABs) are a natural global phenomena emerging in severity and extent. Incidents have many economic, ecological and human health impacts. Monitoring and providing early warning of toxic HABs are critical for protecting public health. Current monitoring programmes include measuring the number of toxic phytoplankton cells in the water and biotoxin levels in shellfish tissue. As these efforts are demanding and labour intensive, methods which improve the efficiency are essential. This study compares the utilisation of a multitoxin surface plasmon resonance (multitoxin SPR) biosensor with enzyme-linked immunosorbent assay (ELISA) and analytical methods such as high performance liquid chromatography with fluorescence detection (HPLC-FLD) and liquid chromatography-tandem mass spectrometry (LC-MS/MS) for toxic HAB monitoring efforts in Europe. Seawater samples (n = 256) from European waters, collected 2009-2011, were analysed for biotoxins: saxitoxin and analogues, okadaic acid and dinophysistoxins 1/2 (DTX1/DTX2) and domoic acid responsible for paralytic shellfish poisoning (PSP), diarrheic shellfish poisoning (DSP) and amnesic shellfish poisoning (ASP), respectively. Biotoxins were detected mainly in samples from Spain and Ireland. France and Norway appeared to have the lowest number of toxic samples. Both the multitoxin SPR biosensor and the RNA microarray were more sensitive at detecting toxic HABs than standard light microscopy phytoplankton monitoring. Correlations between each of the detection methods were performed with the overall agreement, based on statistical 2 × 2 comparison tables, between each testing platform ranging between 32% and 74% for all three toxin families illustrating that one individual testing method may not be an ideal solution. An efficient early warning monitoring system for the detection of toxic HABs could therefore be achieved by combining both the multitoxin SPR biosensor and RNA microarray.</p

Kooistra, Wiebe H C F

Queen's University Belfast Research Portal

https://westminsterresearch.westminster.ac.uk/download/33c6212ef5cdd76434b449985d8404123c408b92265b41cc5f479e1bc6cba845/470781/Final%20HA%20Manuscript%20Feb%2016.pdf

Distribution, occurrence and biotoxin composition of the main

Abstract

Similar works

Full text

Available Versions