Almansa, E. (Eduardo)

Díaz-Barrios, M.C. (María Candelaria)

Garrido, D. (Diego)

López, C.

Marquez, L.

Martín, M.V. (María Virginia)

Miranda, D.

Misol-Rollón, A. (Amador)

Tur, R.

English

Garrido, Diego

Martín, María Virginia

Tur, Ricardo

Miranda, Daniel

Misol-Rollón, Amador

Díaz-Barrios, María Candelaria

Almansa, Eduardo

Digital.CSIC

Effect of different light colors on the Octopus Vulgaris paralarval culture

Tur, R. (Ricardo)

Miranda, D. (Daniel)

Repositorio Institucional Digital del IEO

EFFECT OF DIFFERENT LIGHT COLORS ON THE Octopus vulgaris PARALARVAL CULTURE  D. Garrido1, M.V. Martín1, R. Tur1, D. Miranda1, A. Misol1, M.C. Díaz1, C. López2, L. Marquez3, E. Almansa1  1 Instituto Español de Oceanografía, Centro Oceanográfico de Canarias. Via Espaldón, Dársena Pesquera PCL 8, 38180 Santa Cruz de Tenerife (Spain) 2 Sieltec Canarias SL. C/ Habitat nº 2, Portal D. 38203 La Laguna. Santa Cruz de Tenerife (Spain) 3 Universidad Católica de Temuco. Nucleo de Investigación en Producción Alimentaria. Manuel Montt, 56. Temuco (Chile)  E-mail: eduardo.almansa@ca.ieo.es   Introduction Common octopus (Octopus vulgaris) is an excellent candidate for aquaculture diversification; however, to succeed in its culture it is necessary to overcome the massive mortalities found in paralarvae, which are probably related to zootechnical and/or nutritional aspects. Concerning to zootechnical aspects, light could affect paralarvae performance, since the use of artificial light in the first stages of cephalopod culture, differs in many aspects from the natural light to which paralarvae are biologically adapted. In O. vulgaris paralarvae both natural and artificial light photoperiods have been tested, with light phase duration varying between 12 and 24 hours (De Wolf et al., 2011; Iglesias et al., 2004). Also different illuminance levels have been used, ranging from 60 lx (De Wolf et al., 2011) to 7.500 lx (Okumura et al., 2005), finding different results. Although cephalopods do not distinguish colors (Hamasaki 1968), they can see under a wide light spectrum, even under red light. They have a sophisticated visual system which allows detecting different light features such as brightness, contrast and edges (Aza, 2014). In this sense, the effect of light color on growth, survival or stress response in paralarvae has not been studied. Therefore, the aim of present study is to assess the effect of light colors on growth, survival and corticosterone levels in reared paralarvae.   Materials and methods In the present study, the paralarvae were obtained from wild adult octopus captured in Tenerife coastal waters and maintained in the facilities of the Oceanographic Centre of Canary Islands (Spanish Institute of Oceanography). A total of 1800 hatchlings, at a density of 3 individuals·L-1 (300 paralarvae·tank-1), were reared during 14 days in 100 L black fiberglass cylinder-conical tanks with a flow-through seawater system at 10 mL·s-1 flow (from 18:00h to 8:00h). Paralarvae were fed with 0.5 Artemia·mL-1 (Sep-Art AF INVE Aquaculture, Belgium) enriched with freeze dried Isochrysis galbana (easy algae®, Cádiz, Spain) and Nannochloropsis sp (PhytobloomGreen Formula®, Olhão, Portugal) during 20h after eclosion.  The effects of red-orange vs white light were tested using LED lamps. The measurement of light variables (intensity and spectrum) was carried out using a spectrograph AvaSpec-ULS2048x16. In both groups, intensity was reduced by 75% from 20 pm to 8 am. All treatments were carried out in triplicate. At the end of the experiment, dry weight, specific growth rate and survival were quantified. Samples of 15 paralarvae were collected from each treatment, homogenized and centrifuged to collect the supernatant. Corticosterone levels were determined by radioimmunoassay using an ELISA kit.  Results In experiment 1, non-significant differences in dry weight and specific growth rate (SGR) (Table I) were found. Survival (Figure 1) tended to be significantly higher in paralarvae reared under red-orange light (p=0.071). Corticosterone levels (Table II) did not show significant differences between paralarvae reared with red-orange or white light.   Table I. Dry weight (DW) and specific growth rate (SGR) of 14 days-old O. vulgaris paralarvae reared with red-orange or white light.  Red-orange   White  DW (mg) 0.39 ± 0,01  0.38 ± 0.05  SGR (%  DW·d-1) 4.30 ± 0.09  4.27 ± 0.66  Data are expressed as mean ± standard deviation.    Figure 1. Survival of 14 days-old O. vulgaris paralarvae reared with red-orange or white light. Data are expressed as mean ± standard deviation.   Table II. Corticosterone levels (ng mg prot -1) of 14 days-old O. vulgaris paralarvae reared with red-orange or white light.  Red-orange   White  Corticosterone 37.14 ± 9.22  47.82 ± 8.47  Data are expressed as mean ± standard error.  Discussion and conclusion In the present study the survival tended to differ between treatments. Therefore, our results suggest that red-orange light may have an effect on the survival of Octopus vulgaris paralarvae and could improve the culture of paralarvae of this species. However, more studies about the effect of other light spectra on the culture of this species paralarvae are necessary to confirm these tendencies.  References Aza Aquatic invertebrate taxon advisory group (AITAG). 2014. Giant pacific octopus (Enteroctopus dofleini) care manual. Association of zoos and aquariums, Silver Spring, MD. De Wolf, T., S. Lenzi, and F. Lenzi. 2011. Paralarval rearing of Octopus vulgaris (Cuvier) in Tuscany, Italy. Aquaculture Research 42(9), 1406-1414. Hamasaki, D. I. 1968. The ERG-determined spectral sensitivity of the Octopus. Vision Research 8: 1013-1021. Iglesias, J., J.J. Otero, C. Moxica, L. Fuentes, and F.J. Sánchez. 2004. The completed life cycle of the octopus (Octopus vulgaris, Cuvier) under culture conditions: paralarval rearing using Artemia and zoeae, and first data on juvenile growth up to 8 months of age. Aquaculture International 12(4-5), 481-487. Okumura, S., Kurihara, A., Iwamoto, A., Takeuchi, T., 2005. Improved survival and growth in Octopus vulgaris paralarvae by feeding large type Artemia and Pacific sandeel, Ammodytes personatus. Improved survival and growth of common octopus paralarvae. Aquaculture 244, 147-157. 0102030405060Survival (%)Red-orangeWhite

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Effect of different light colors on the Octopus Vulgaris paralarval culture

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