Navarro, M.R. (María Rosario)

Silva, Alexandra

Silva, Andreia

Soares, Eduardo

Villamor, B. (Begoña)

English

Navarro, María Rosario

Villamor, Begoña

Digital.CSIC

Summary of the different techniques of chub mackerel otoliths preparation by laboratory

Silva, A. (Andreia)

Soares, E. (Eduardo)

Silva, A. (Alexandra)

Repositorio Institucional Digital del IEO

1Workshop on Age Reading Of Chub Mackerel(Scomber colias) [WKARCM]ICESLisbon, 02-06 November 2015Summary of the different techniques of chub mackerel otoliths preparation by laboratoryAndreia V. Silva Maria Rosario NavarroBegoña VillamorEduardo SoaresAlexandra SilvaInstitutions participants2GalicianWaters / Bay of Biscay IXaN, VIIIc, VIIIb and VIIIaMauritanianWatersCECAF MauritaniaLigurian andnorthTyrrhenian seaGFCM - GSA9PortugalIXaN, IXaSWestern Mediterranean GSA6Central Southern Tyrrhenian Sea, Southern Adriatic SeaGSA 10, GSA 18, GSA 19AreaFAO/ICESAreaCanaria Islands SantanderMurciaCN1Diapositiva 2CN1 We have to add the information about IEO-Murcia and COISPA-Italy.IEO-Murcia: Western Mediterranean, GSA6COISPA-Italy: Central Southern Tyrrhenian Sea (GSA 10), Southern Adriatic Sea (GSA 18) and West Ionian Sea (GSA 19) Charo Navarro, 28/10/2015Calcified structures3Whole otoliths mounted in Eukitt® resin in black platic platesBlack plateswith cover, inside a cardboard boxWhole otoliths mounted in Resin in black plastic platesBlack plateswith cover, inside a cardboard boxWhole otoliths immersed in sea waterDryed andstored in a plastic phialWhole otoliths mounted in Entellan® resin in black plastic platesBlack plateswith cover, inside a cardboard boxWhole otoliths mounted in clear resin in black plasticslidesPlastic bagsWhole otolithsimmersed in sea waterDryed andstored in a plastic box PreparationMountedStoredCanaria Islands SantanderMurcia4Image AnalysisNis-ElementsViewer 4.0Color Digital Camara. Nikon Sight. DS-5MpixelsNis-Elementand ImageProNikon Digital Sight Ds-U2Image Pro Premiere 9Digital camera: color CCD, resolution1295 x 960 pixelVisiolog6.3/TNPC 4Sony digital cameraDFY-SX910; 1,45 MpixelsIrfan view (analysis); Las ez as (capture)Digital cameraImage JBELL DV1300 CMOS CameraSoftwareCameraCanaria Islands SantanderMurcia5Images AnalysisExchanges, validation studies, publications, thesis, an age determination procedures manual  elaboration, resolution of doubst and an otolith image bankOtolithdiameter/radius, annual ring radius, AMD, RMDExchanges, annualperiodicity, validation for ring deposition, backcalculationDistancebetweenrings, otolith radius andannual ring radiusRoutine, exchanges, validationstudies, thesisRadios, lenght, distancebetween rings, annual ring radiusExchanges, ThesisAnnual ring radius anddiameter, otolith radiusand diameterExchangesDistance between nucleus and first and second ring.Exchanges, validation studies and formation a reference collectionOtolith total length, radius and ring radiusUsageMeasuresCanaria Islands SantanderMurcia6Images Analysis±300 imagesCalibrated, LIM; JPEG; TIFF; PSPNot routinelyCalibrated, TIFF or JPEG±500 imagesNot calibrated, JPEGNot routinelyCalibrated, TIFF or JPEGNot routinelyCalibrated, TIFF or JPEG30Calibrated, JPEGCalibration/FormatN. Images/yearCanaria Islands SantanderMurcia7SummaryCountry Institute Laboratory Calcified pieces Preparation Stored beforetreatmentStored aftertreatmentSpain IEOInstituto Españolde Oceanografia –CanáriasWhole otoliths Otoliths mounted in clear resin in black plastic platesCleaned and dried, in labelled plastic containersLabelled in black plastic plates Italy CIBMCentro Interuniversitario Biologia Marina “G.Bacci”Whole otoliths Otoliths inmersedin sea waterDried and stored in a pvc phial.Portugal IPMAInstituto Português do Mar e AtmosferaWhole otolithsOtoliths mounted in resin (Entellan®, Merck, ref. HX807787) in black plastic platesCleaned and dried in epandorffsLabelled in black plastic plates Spain IEOInstituto Españolde Oceanografia –SantanderWhole otolithsOtoliths mounted in resin (Eukitt®) in black plastic plates with coverLoose and dry inside labelled plastic plates with cover, in a horizontal positionFixed in resin in black plastic plates with cover; stored in cardboard boxes in the laboratoryItaly COISPAStazione Sperimentale per lo studio delle rosorse del mareWhole otoliths Otolith immersed in the clarification medium (sea water)Dried in the plastic boxAfter the age analysis the otolith are dried again and stored in the same boxSpain IEO Murcia whole otoliths otoliths mounted in clear resin on custom plastic slidesThey are directly mounted in clear resin on custom plastic slides.In plastic bags.8SummaryCountry Institute Image capture software Type of camera Images usesSpain IEO – Canaria IslandsNIS - Elements and Image Pro. Nikon Digital Sight DS-U2.Exchanges, annual periodicity validation for one ring deposition, backcalculation.Italy CIBM Image pro premiere 9.1Digital camera: color CCD sensor 1/3”, resolution 1295 x 960 pixel with 8/12 bit color (mod. C125)Routine, exchanges, validation studies, thesisPortugal IPMA Visilog 6.3/ TNPC 4 Sony digital camera DFY-SX910 Exchanges, thesisSpain IEO –Santander NIS-Elements Viewer 4.0Color Digital Camera. NIKON Digital Sigth. DS-5MExchanges, validation studies, publications, thesis, age determination procedures manual, resolution of doubts, otolith image bankItaly COISPA ImageJ BELL DV1300 CMOS Camera Exchanges, validation studies and formation a reference collectionSpain IEO - Murcia Irfan view (analysis); Las ezas (capture) digital camera Exchanges9Country Institute Image measurements type Number images per year Calibrated images Image formatSpain IEO – Canarias IslendsDistance between rings,otolith radius and annualring radius.Not routinely Yes. TIFF or JPEG.Italy CIBMRadius, length, distancebetween rings, annual ringradiusAbout 500 No JPEGPortugal IPMA Annual ring radius, otolithradius Not routinely Yes. TIFFSpain IEO -SantanderOtolith diameter, otolithradius, annual ring radius,absolute marginaldistance, relative marginaldistance200-300 Yes. LIM images, JPEG, TIF, pspItaly COISPA Otolith total length, radiusand ring radius 30 yes. JPEGSpain IEO - Murcia Distance between nucleusand first and second ring. Not routinely yes JPEGSummary

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Summary of the different techniques of chub mackerel otoliths preparation by laboratory

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