Linares, F.

Olmedo-Herrero, M. (Mercedes)

Peleteiro-Alonso, J.B. (José Benito)

English

Linares, Fátima

Olmedo-Herrero, Mercedes

Peleteiro-Alonso, José Benito

Digital.CSIC

Biochemical coposition of blackspot seabream larvae, Pagellus Bogaraveo, fed with rotifer enriched with three different microalgae

Linares, F. (Fátima)

Repositorio Institucional Digital del IEO

l..TURE INciences, 1061 China andechniques ofre are given,1 sustainableBIOCHEMICAL COMPOSITlON OF BLACKSPOT SEABREAM LARV AE,PAGELLUS BOGARA VEO, FED WITH ROTIFER ENRICHED WITH THREEDIFFERENT MICROALGAEF. Linares', M. Olmedo/ and J.B. Peleteiro"'Centro de lnvestigacións Mariñas s/n, E-36620. Vilanova de Arousa (Pontevedra),Spain, E-mail: flinares@cimacoron.org2Instituto Español de Oceanografía, Cabo Estay-Canido, Apartado 1552, 36280 Vigo(Pontevedra), SpainIntroductionBlackspot seabream, Pagellus bogaraveo, is one of the farmable species at cornmercialleve!. The success of larvar rearing is greatly influenced by first feeding regimes and thenutritional quality of starter diets (Izquierdo et al.,2000). Obtaining larvae with a suitablebiochemical composition is the result of good food and is essential to obtain good qualityyoung individuals. This work studies the biochemical composition of blackspotseabream larvae at the feeding stage, fed with rotifer enriched with three differentmicroalgae, Isochrysis galbana, Tetraselmis suecica and Nannochloropsis sp2.Materials and MetbodsThe experiment was conducted with blackspot seabream larvae from spawns obtainedduring the year 2000 from the broodstock belonging to the l.E.O. in Vigo and CIMA(Local Govemment of Galicia). Three samples of larvae were taken at the start (day O)and at the end of the experience (day 15 of life). Analyses were performed on proteins(Bradford, 1976), total lipid by extraction with chloroform: methanol (2: 1) (Blight &Dyer, 1959 modified by Femández Reiriz et al. 1989) and gravimetric determination.Fatty acids prior to transesterification and metilation (Lepage & Roy, 1986) wereanalyzed by Gas Cromatography.Results and DiscussionTable 1 shows the protein, lipid and fatty acid composition in blackspot seabream larvae.Protein values ranged from 47% in recently hatched larvae, to 49, 48 and 53% in larvaefed with rotifer enriched with Isochrysis, Tetraselmis and Nannochloropsis respectively.Considerable lipid consumption was noted in the initial 15 days of larvae life, fallingfrom the 26% achieved in recently hatched larva e to values close to 15%. The constantvalues in proteins and the decrease in total lipids throughout larval development inblackspot seabream was noted down previously (Linares et al, 2000). No significantdifferences were noted in the composition of lipids and proteins in larvae fed with rotiferenriched with the three three mentioned microalgae. The PUFA percentage in terms ofthe total amount of fatty acids varied frOID39% in recently batched larvae to 37,34 and30138% in 15 day oId larvae enriched with Isochrysis, Tetraselmis and Nannochloropsisrespectively.Table L Proteins, lipids (% of dry weight) and fatty acids (% of total) in larvae ofblackspot seabream newly hatched and fed with rotifer enriched with microalgae.Larvae newly 15d. larvae fed 15 days larvae 15 days larvaehatched withR+Iso fed with R+Tetra fed withR+NannoProteins 47.29 48.86 48.07 53.19Lipids 25.8 15.2 14.53 15.29PUFA 38.92 36.63 33.57 37.73Saturates 28.03 33.97 34.97 35.98Monoinsaturates 26.74 20.30 21.16 18.29Sumo(n-3) 32.74 22.05 25.81 27.29Sumo(n-6) 5.57 13.11 6.52 8.4220:5(n-3).EPA 6.86 2.34 9.84 11.7122:6(n-3) DHA 22.55 15.79 6.86 6.21Significant differences were noted between the docosahexaenoic fatty acid content,22:6(n-3), among larvae fed with rotifer enriched with Isochrysis, which accounted for16% of the total of fatty acids, in contrast with the 6.8 and 6.2% obtained in larvae fedwith rotifer enriched with Tetraselmis and Nannochloropsis respectively. This may berelevant in view ofthe considerable importance ofthis fatty acid in marine fish.ReferencesBlight, E.G. & Dyer, W.J.1959. A rapid method of totallipid extraction and purification. Can.J.Biochem, Physiolog., 37:911-917.Bradford, M.M. 1976. A rapíd sensitive method for the quantitation of microgram quantities ofprotein utilizing the principle ofprotein-dye binding. Analytical biochemistry 72, 248-254.Fernández-Reiriz, M.J., Perez-Carnacho,A., Ferreiro,M.J.,Blanco,J., Planas,M. Campos,M.J.&Labarta,U. 1989. Biomass production and variation in the biochemical profile(totalprotein, carbohydrates, RNA, lipids and fatty acids) of seven species of marinemicroalgae. Aquaculture,83:17-37.Izquierdo, M.S., J.Socorro,L. Arantzamendi & C.M.Hernández Cruz.2000. Recent advances inlipid nutrition in fishes. Fish Physiology and biochemistry 22:97-107.Linares,F., Olmedo, M., Peleteiro, J.B. & Gianzo,C. 2000. Biochemical composition ofblackspotseabream larvae, Pagellus bogaraveo, throughout larval development. Initial results. Europeanaquaculture Society. Special publication n°28:400.302Tl11Li:y{NaAlTubecFis19'toint.ChmaAtPrcwaseaturlFOJcarpre.,r~' rec.~.:; mosubKe:turl

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Biochemical coposition of blackspot seabream larvae, Pagellus Bogaraveo, fed with rotifer enriched with three different microalgae

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