Acosta, N.G.

Cejas, J.R. (Juana Rosa)

Corriero, A.

Herrera, J.

Jerez, S. (Salvador)

Lorenzo, A.

Rodriguez, C.

Rodríguez-Barreto, D.

English

Acosta, Nieves

Jerez, Salvador

Cejas, Juana Rosa

Digital.CSIC

FATTY ACID PROFILE OF WILD GREATER AMBERJACK FEMALE GONADS FROM MEDITERRANEAN AND ATLANTIC AREAS

Acosta, N.G. (Nieves)

Repositorio Institucional Digital del IEO

FATTY ACID PROFILE OF WILD GREATER AMBERJACK FEMALE GONADS FROM MEDITERRANEAN AND ATLANTIC AREAS   J. Herrera1, D. Rodríguez-Barreto1, N.G. Acosta1, A. Corriero3, S. Jerez2, J.R. Cejas2, A. Lorenzo1and C. Rodriguez1*  1Universidad de La Laguna. E-mail:covarodr@ull.es 2Centro Oceanográfico de Canarias, Instituto Español de Oceanografía 3Universitadeglistudi di Bari "Aldo Moro"   Introduction The lack of reliable reproduction is one of the main bottlenecks for the incorporation of greater amberjack (Seriola dumerili) in the EU aquaculture industry. In the frame of the FP7-funded DIVERSIFY, to assess the reproductive potential of wild vs. captive amberjack broodstocks and identify possible reproductive/metabolic dysfunctions during gametogenesis are important goals. In this sense, characterization of wild broodstock nutritional status is crucial. Greater amberjack, like all other marine carnivorous fish studied so far, requires eicosapentaenoic acid (20:5n-3, EPA), docosahexaenoic acid (22:6n-3, DHA) and arachidonic acid (20:4n-6, AA) for reproduction success. Female gonad proportions of phosphatidylcholine (PC) and phosphatidylethanolamine (PE) and their fatty acid (FA) composition are critical factors to allow proper reproductive function and embryonic development. The importance of n-3 HUFA and particularly DHA content on gonadal development and egg quality (fecundity, hatching and larvae survival rates) has been highlighted by several authors. In addition, AA eicosanoids derivatives have a wide range of functions in fish reproduction, including pheromonal attraction, steroidogenesis, or ovulation and oocyte maturation. Since EPA and AA compete for the same enzymatic complex to generate different series of prostanoids, the relative proportions of these fatty acids in broodsctock gonads seem to be more important than the level of each fatty acid so that imbalances in the EPA/AA ratio could lead to deregulated production of different mediators involved in reproduction.  Fatty acid composition of fish tissues is mainly influenced by dietary fatty acid profile and environmental factors including temperature. Therefore, considering that Mediterranean and Atlantic broodstocks will be studied within the context of DIVERSIFY, the aim of this study was to compare the total lipid (TL) content and FA profiles from ovaries of wild Atlantic and Mediterranean mature females of greater amberjack.  Material and Methods The TL content and ovary fatty acid composition of TL extract and PC, PE and triacylglyceride (TG) fractions from 5 wild Atlantic mature females (wt 14.4±5kg; GSI 3.5±0.8) and 6 wild Mediterranean mature females (wt 12.1±1kg; GSI 4.5±1.2) were statistically compared.  Results No significant differences were found in terms of ovary TL between fish from both geographical areas (16.6±1.1 and 17.8±0.8, respectively). Mediterranean fish displayed a higher level of saturated fatty acids, C16:1, EPA and AA whereas Atlantic females were richer in 18:1n-9. Results from the principal component analysis (PCA) (Figure 1) show that Mediterranean and Atlantic populations can be clearly distinguished based on the ovary whole fatty acid profiles of TL, and PC,PE and TG fractions. Despite the above mentioned differences in the levels of EPA and AA, non geographical differences were found in terms of ovaries EPA/AA ratios and DHA total contents.   Fig.1.PCA of fatty acid profiles of TL, and PC,Atlantic and Mediterranean individual scores for each PPC1 according to T-student Discussion and conclusionIt is worthy to notice that the can be useful to identify their geographical origin, accordingly to acids previously reported for other vertebratesthe EPA/ARA ratio and DHA conlipidic features unaltered Barreto et al. 2014).  Acknowledgments This project has received funding from the European Union’s Seventh Framework Programme for research, technological development and demonstration (KBBEsingle stage, GA 603121, DIVERSIFY).Degree Thesis on Marine  References Rodríguez-Barreto, D., S. Jerez, J.R. Cejas, 2014.Ovary and egg fatty acid composition of greater amberjack broodstock (dumerili) fed different dietary fatty acids profiles. European Journal of Lipid Science and Technology 116(5): 584Lister, A. L., and Van Der Kraak, G, 2008. oocyte maturation and ovulation.General and comparative endocrinology 159(1): 46 PE and TG fractions of ovarwild females. Factor score plot (graphical representation of rincipal Component). Ellipses represent different clusters for  results; [(○) Atlantic females; (○) Mediterranean femaless ovary whole FA profile of the different femalestraceability. In spite of this, the lack of differences in tents, confirms the importance of maintaining this two to ensure reproductive success (Lister et al. 2008; Part of these results belongs to the Master Biology of J. Herrera. V.M. Martin, N.G. Acosta, A. Bolaños, and A. Lorenzo, -595. An investigation into the role of prostaglandins in zebrafish ies from ].  analyzed  of fatty Rodriguez--2013-07 Seriola -57. 

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FATTY ACID PROFILE OF WILD GREATER AMBERJACK FEMALE GONADS FROM MEDITERRANEAN AND ATLANTIC AREAS

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