Albentosa-Verdú, M. (Marina)

Bellas, J. (Juan)

Campillo-González, J.A. (Juan Antonio)

González-Fernández, C. (Carmen)

Viñas, L. (Lucía)

English

Albentosa, M. (Marina)

Repositorio Institucional Digital del IEO

This study has been funded by the BIOCOM project (CTM2012-30737) funded through the Spanish National Plan R&D (2008-2011). C. González-Fernández was supported by a pre-doctoral IEO grant. We are grateful to technical personnel  of Marine Pollution Group at the IEO-Murcia & IEO-Vigo.  3. Results and Discussion M Albentosa1, C González-Fernández1 , JA Campillo1, L Viñas2 , A. Franco2, J Bellas2   1Instituto Español de Oceanografía, Centro Oceanográfico de Murcia, Varadero, 1, 30740 San Pedro del Pinatar, Murcia, Spain.   2 Instituto Español de Oceanografía, Centro Oceanográfico de Vigo, Subida a Radio Faro 50, 36390 Vigo, Spain   Effect of mussel size on the toxicity of Fluoranthene 2. Materials and Methods  Physiological rates (standardized to a common size) were size-dependent with decreases at higher mussel sizes.  Higher feeding/absorption rates in smaller mussels were compensated with higher respiration rates, thus estimated growth is independent of size.  Smaller mussels (S & M) were more sensitive to FLU than large mussels.  The effect of FLU exposure was the reduction of the energy available for growth  Endogenous factors, such as body size, need to be considered in toxicological studies and biomonitoring programs. 4. Conclusions carmen.gonzalez@mu.ieo.es CONDITIONING 1. Introduction There are several endogenous and exogenous variables that act as confounding factors making the environmental assessment harder. Among these endogenous factors, body size has been considered as a variable which influences pollutant bioaccumulation and biomarker responses. The size of animals with unlimited growth as mussels, is closely related with their age, therefore the size-dependence of these biological processes could also be related to age.        OBJECTIVE Evaluate the effect of mussel size on the toxicity of the polycyclic aromatic hydrocarbon (PAH) fluoranthene (FLU), measured by means of the physiological rates  integrated in the energy balance equation used for the SFG (scope for growth) estimation. S-Small (30 mm) M-Medium (40 mm) L-Large (50 mm) •The integration of all these parameters in the SFG showed that S and M mussels were impacted by FLU exposure whereas L mussels were not.  Total  Gill  Mantle  Gland  Rest  160.8 17.9 15.4 30.2 94.3 Total  Gill  Mantle  Gland  Rest  340.7 44.9 51.7 58.5 179.3 Total  Gill  Mantle  Gland  Rest  578.8 89.3 103.3 97.4 277.4 S M L S Control  S FLU M Control  M FLU L Control  L FLU PHYSIOLOGICAL BIOMARKERSC = F + U + R + PEnergyconsumedEnergy lost in faecesEnergy due toexcretion Energy lossesdue torespirationPRODUCTIONScope for growthSomatic growth ReproductionSFGExposure Conditions: •30 µg FLU/L daily •3 weeks •Daily food ration (t-ISO) 0.15 % mussel live weight •17ºC  Standardized Conditions: •17ºC •0.6 mg OM/L (t-ISO) •0.4 mg IM/L (ashed silt) aA bB a aB a a 1.5 2.0 2.5 3.0 3.5 4.0 Small Medium Large CR (L/ind st/h) Control FLU 20 25 30 35 40 45 50 55 Small Medium Large AR (J/ind st/h) •Clearance rates (CR) were higher in S and M mussels and the effect of FLU was only evident in S mussels.  •Absorption rates (AR) were clearly affected by size (higher in smallest mussels) and by FLU: in S and M mussel, ARs were reduced by exposure whereas no difference was detected in L mussels.  bB a bAB a a aA 0 5 10 15 20 25 Small Medium Large RR (J/ind st/h) aC a a a aB aA •Respiration rates (RR) were only affected by size, with higher values in smallest mussels, whereas FLU exposure did not alter RRs. 5 10 15 20 25 30 35 Small Medium Large SFG (J/ind st/h) a bAB bB aA a a Two-way ANOVA Fsize FFLU Fsize*FLU Clearance 5.52  (p<0.01) 8.02 (p<0.01) 1.55 (p>0.05) Absorption 7.52  (p<0.01) 15.68 (p<0.01) 4.51 (p<0.01) Respiration 271.67 (p<0.01) 1.34 (p>0.05) 0.64 (p>0.05) Growth 0.20 (p>0.05) 15.41 (p<0.01) 4.55 (p<0.01) •All the physiological rates were clearly influenced by size , showing  decreases in  clearance, absorption and respiration  (standardized to a common size) as size increases.  •Respiration was the physiological parameter most  affected by size.  •The integration  of energy inputs  and outputs into the SFG, removes the effect of size on physiological rates.  •FLU exposure reduces  clearance and absorption rates.  •FLU  exposure  does not affect respiration rates.  •FLU exposure reduces mussel growth but only in S and M mussels.  

Effect of mussel size on the toxicity of fluoranthene

Albentosa, Marina

González-Fernández, Carmen

Viñas, Lucía

Campillo-González, Juan Antonio

Bellas, Juan

Digital.CSIC

http://www.repositorio.ieo.es/e-ieo/bitstream/handle/10508/9550/Albentosa%20et%20al_PRIMO%2018.pdf?sequence=2&isAllowed=y

Effect of mussel size on the toxicity of fluoranthene

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