Consiglio, O

LIBRIZZI, Mariangela

LONGO, Alessandra

LUPARELLO, Claudio

Tobiasch, E

Archivio istituzionale della ricerca - Università di Palermo

i;i;,1/ P29 . EFFECT OF CONDITIONED MEDIA FROM OSTEO- AND ADIPO.DTFFERENTIATING MESENCHYMAL STEM CELLS ON TRIPLE NEGATIVE MDA.M8231 BREAST CANCER CELLSM. Librizzi', A. Longol, O. Consigliol, E. Tobiasch2, C. Luparelloll.Dipartimenro STEBICEF, Università di palermo,2'Department of Natural Sciences, University of Applied Science, Bonn-Rhein-Sieg (D)*Corresponding author: Prof. Claudio Lupareìio: Dipartimento di Scienze e TecnologieIt"l"F:h_", Chimiche e Farmaceutiche. Univeìsità degli Studi di palermo, Viale delle ScieùeEd. 1 6, Palermo, Italy tel : 09 L23997 405 e-mail: claudio.luparello @unipa.it\.It is ftrow that mesenchymal stem cells (MSCs) actively secrete multiple biologically-activefactors during their process of differentiation which gives rise to a variey of "ytotypes includingbone and fat cells' It is also acknowledged that the chemokines secreted ttràughout MSòdifferentiation may play an important role in the development and growth of ùmor cells,although iiterature data appear somewhat indeterminate due to the contradictory evidence oftenfound [1].The purpose of this study was to evaluate the effect of conditioned media (CMs) from MSCs,cultured fot 7, 14,2I and 28 days in osteo-, adipo-differentiating and undifferentiated conditions,on MDA-MB231 breast cancer cells, an "in vitro" model systan derived from a triple-negativebreast cancer (TNBC). MTT assay showed that the CMs collected after 28 days of both òsteo-and adipo-differentiation induced growth inhibition on MDA-M8231 cells after 24 h ofincubation. In light of such evidence, these CMs were used to treat cells and performcltofluorimetric assays to better evaluate their biologicai effects on viability/proliferati,on, ceilcycle progression, apoptosis/autophagy induction and mitochondrial activitylreactive oxygenspecies (ROS) accumulation of MDA-MB231 cells.The most interesting results regard the ability of CMs from osteo-differentiating MSC to inducean alteration of cell proliferation with an arrest in the GzlM transition phase of the cycle coupledto both apoptotic and autophagic promotion. No accumulation of ROS and impairmeni ofmitochondrial respiration was observed at the end of treatment. On the other hand, preliminaryindications suggest that the CMs isolated from adipo-differentiating MSCs have different effectfrom those obtained by osteo-differentiating cultuies, being the lethality unlinked to apoptosisand autophagy, and thereby prompting to get more insight into the anti-TNBC activity strown Uythe different CMs at the molecular level.[1] Alan PM, Fiachra TM, Roisin MD, Tomas PG, Mary M, Frank pB, Timothy O and MichaelJK' Mesenchymal stem cell secretion of chemokines duiing differentiation into osteoblasts, andtheir potential role in mediating interaction with breast cancer cells. Int. J. Cancer 2009;124:326-332.This work was supported by University of Palermo (R.S. ex60Vo andFFR 2013) and progertoVigoni 2011 for Claudio Luparello, and BMBF, AdipaD, 1120x06, FHprofUnt, FKZ:03FH012P82; FH-Extra,FKZ: zlll2fhAl}; DAAD, PPP Vigoni,FKZ: 5466921g;BMBF-AIF,FKZ: ll20X06; and Geràteprogramm, Fachhochschulen NRW 2008 for Edda Tobiasch.100

Effect of conditioned media from osteo- and adipodifferentiating mesenchymal stem cells on triple negative MDA-MB231 breast cancer cells

https://iris.unipa.it/retrieve/handle/10447/86706/93227/abstract%20SIBS%202.pdf

Effect of conditioned media from osteo- and adipodifferentiating mesenchymal stem cells on triple negative MDA-MB231 breast cancer cells

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Archivio istituzionale della ricerca - Università di Palermo