Carrozzo, A

Di Marco, V.

Piraino, C

Scatassa, L

SCHILLACI, Domenico

Sciurba, F

Vicari, D

Vitale, M

Archivio istituzionale della ricerca - Università di Palermo

3715 PRELIMINARY COMPARISON FOR GENES INVOLVED IN BIOFILMS IN COAGULASE-NEGATIVE STAPHYLOCOCCI (CONS) AND S. AUREUS STRAINS ISOLATED FROM MILK SAMPLES OF SICILIAN FARMS M. Vitale1, L. Scatassa2, D. Vicari3, A. Carrozzo2, F. Sciurba4, C. Piraino4, D. Schillaci5, V. Di Marco Lo Presti6 1Molecular Biology, 2Area Assistenza Produzioni, 3Area Palermo, 4Microbiology, Istituto Zooprofilattico Sperimentale of Sicily A. Mirri, 5Dept. STEBICEF, Università degli Studi di Palermo, Palermo, 6Area Barcellona Pozzo di Gotto, Istituto Zooprofilattico Sperimentale of Sicily A. Mirri, Barcellona Pozzo di Gotto, Italy Mastitis in small ruminant farms are quite spread in Sicily. Coagulase-negative staphylococci (CoNS) are frequently isolated from milk of animals with sub-clinical mastitis. They are less pathogenic compared to S.aureus but can cause persistent infections with increased somatic cell count (SCC) in milk and consequentially reduction of milk quality. Bacteria organized in biofilm communities could be responsible for the persistency of infection.   Sicilian sheep flocks interested in clinical and sub-clinical mastitis were selected to isolate bacteria strains from milk samples. We started a comparison of S.aureus and CoNS isolates for the presence of bac and ica genes and the evaluation of their capability in biofilm formation.   The Bacteria were isolated from bulk milk samples. The isolated colonies were typed by API Staph strip ( BioMerieux). PCR was performed with primers for ica and bap genes. The analysis for biofilm formation was performed by safranin method in microtiter plates.  The results showed that intercellular adhesion (ica) operon was present in many isolates of both S.aureus and CoNS (circa 85%) whereas the biofilm-associated protein bap gene was present at higher percentage ( 43%) compared to the S.aureus isolates (8%).   A relation on ica operon presence and biofilm formation seems to be present for S. epidermidis and not for S. aureus but analysis in a larger number of isolates of both coagulase positive and negative staphylococci will be performed.   The technical contribution of Dr. La Giglia is highly appreciated. The work is granted by RCSI 2010 to M. Vitalee.   

PRELIMINARY COMPARISON FOR GENES INVOLVED IN BIOFILMS IN COAGULASE-NEGATIVE 
STAPHYLOCOCCI (CONS) AND S. AUREUS STRAINS ISOLATED FROM MILK SAMPLES OF SICILIAN FARMS

https://iris.unipa.it/retrieve/handle/10447/96714/89900/3715.pdf

PRELIMINARY COMPARISON FOR GENES INVOLVED IN BIOFILMS IN COAGULASE-NEGATIVE STAPHYLOCOCCI (CONS) AND S. AUREUS STRAINS ISOLATED FROM MILK SAMPLES OF SICILIAN FARMS

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Archivio istituzionale della ricerca - Università di Palermo