CAVALIERI, Vincenzo

Archivio istituzionale della ricerca - Università di Palermo

 	   Vincenzo Cavalieri  University of Palermo  The Ambulacraria-specific COMPASS-like protein contributes to sns5 chromatin insulator function in the sea urchin embryo  The sea urchin chromatin insulator sns5 lies within the early histone gene cluster, basically between the H2A enhancer and H1 promoter. The COMPASS-like (CMPl) protein, which is expressed exclusively by the ambulacrarian group of metazoans, represents the first insulator binding protein so far identified in echinoderms. Specific association of CMPl with sns5 has been demonstrated by using a yeast one-hybrid system, and further corroborated by ChIP-qPCR and trans-activation assays in developing embryos. Challenging CMPl function, either by injecting into zygotes a purified CMPl antibody or expressing the DNA-binding domain of CMPl, impaired the enhancer-blocking function of sns5, as indicated by the specific increase of the H1 expression level. Furthermore, coinjection of the CMPl antiserum and a synthetic mRNA encoding a forced repressor of the H2A enhancer-bound factor restores the normal H1 mRNA abundance. Altogether, these results strongly support the conclusion that the recruitment of CMPl on sns5 is required for buffering the H1 promoter from the H2A enhancer activity, and this, in turn, accounts for the different level of accumulation of linker and nucleosomal transcripts.  November 27 , 17:30 Institute for Genetics, Zülpicher Str. 47a, Lecture Hall, 4th Floor  Hosts: Andreas Beyer and Thomas Wiehe www.sfb680.uni-koeln.de  

The Ambulacraria-specific COMPASS-like protein
contributes to sns5 chromatin insulator function in the sea urchin embryo

https://iris.unipa.it/retrieve/handle/10447/84877/95152/86_Vincenzo%2520Cavalieri.pdf

The Ambulacraria-specific COMPASS-like protein contributes to sns5 chromatin insulator function in the sea urchin embryo

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Archivio istituzionale della ricerca - Università di Palermo