AGNELLO, Maria

FILOSTO, Simone

ROCCHERI, Maria Carmela

TRINCHELLA, F

Archivio istituzionale della ricerca - Università di Palermo

PRINT  CLOSE WINDOW   FISV 2008 - 10th Annual Congress Riva del Garda, 24-27 September 2008 http://fisv2008.azuleon.org   Cellular stress, apoptosis and autophagy 25/09/2008, 08:30 - [---] (Organisers: T. Pozzan, M. Piacentini)   Abstract UAI: fcbc68ea0630150557   Stress induced and physiological apoptosis during early development of sea urchin Paracentrotus lividus  M. Agnello , S. Filosto , F. Trinchella , M.C. Roccheri  Dip. Biologia Cellulare e dello Sviluppo "A. Monroy", Univ. Palermo (Palermo); Univ. Federico II, Dip.di Scienze Biologiche, Sezione di Biologia Evolutiva e Comparata (Naples)   We investigate the toxic effect of cadmium on P. lividus embryo development. We already ascertained that 1mM and 100µM CdCl  induce apoptosis and found a competition between Cd and Ca uptake during early development. Using calcein-AM we show that intracellular amount of Ca in Cd-treated embryos is effectively lower than controls. We find cadmium to induce apoptosis through caspase-3 activation, while caspase-7 is the main effector of physiological apoptosis. Caspase-10 results active only in physiological apoptosis, since caspase-8 is predominantly involved in stress-induced apoptosis. Another stress inducer, the TPA, causes activation of all examined caspases. Immunocytochemical experiments with anti-cleaved-caspase-9 antibody, immunoblotting with anti- cytochrome-C antibody and fluorescent assays with JC-1 fluorochrome fail to find a mitochondrial involvement in apoptosis. Moreover, we show a detectable generation of reactive oxygen species, following exposure to Cd. We studied also caspases expressions during early physiological development and demonstrate that caspase-3 and caspase-10 are well-represented only after mesenchyme blastula stage. 1 1 2 11 22Pagina 1 di 1Abstract17/02/2010http://fisv2008.azuleon.org/ShowAbstractOnline.php?uai=fcbc68ea0630150557

Stress induced and physiological apoptosis during early development of sea urchin Paracentrotus lividus.

https://iris.unipa.it/retrieve/handle/10447/36621/15796/__fisv2008.Fisv%202008%20Agnello.pdf

Stress induced and physiological apoptosis during early development of sea urchin Paracentrotus lividus.

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Archivio istituzionale della ricerca - Università di Palermo