none8This study describes the effects of a static magnetic field (SMF) on cell growth and DNA integrity of
human umbilical vein endothelial cells (HUVECs). Fast halo assay was used to investigate nuclear
damage; quantitative polymerase chain reaction (QPCR), standard PCR, and real-time PCR were used
to evaluate mitochondrial DNA integrity, content, and gene expression. HUVECs were continually
exposed to a 300mT SMF for 4, 24, 48, and 72 h. Compared to control samples (unexposed cultures)
the SMF-exposed cells did not show a statistically significant change in their viability. Conversely, the
static field was shown to be significant after 4 h of exposure, inducing damage on both the nuclear and
mitochondrial levels, reducing mitochondrial content and increasing reactive oxygen species. Twentyfour
hours of exposure increased mitochondrial DNA content as well as expression of one of the main
genes related to mitochondrial biogenesis. No significant differences between exposed and sham
cultures were found after 48 and 72 h of exposure. The results suggest that a 300mT SMF does not
cause permanent DNA damage in HUVECs and stimulates a transient mitochondrial biogenesis.
BioelectromagneticsopenPOTENZA L; MARTINELLI C; POLIDORI E; DONATI ZEPPA, S; CALCABRINI C; STOCCHI L; SESTILI P; STOCCHI V;Potenza, LUCIA ANNA MARIA; Martinelli, Chiara; Polidori, Emanuela; DONATI ZEPPA, Sabrina; Calcabrini, Cinzia; Stocchi, L; Sestili, Piero; Stocchi, Vilbert