The precise, systematic, explicit, particular, linear, exact and robust scientific method was developed and validated for the assay of Triflumezopyrim in SBT TRIFLUMEZOPYRIM 10 SC fungicide. Presently utilized Triflumezopyrim as a working standard having limit for assay of Triflumezopyrim in SBT TRIFLUMEZOPYRIM 10 SC (CILPYROX) fungicide are not less than 95.0%. Acetonitrile, water in the ratio (70:30 v/v) used as mobile phase and flow rate 1.0 ml / min. with 15 minutes run time. The detection was carried at 265 nm with column c18 - 250mm x 4.6mm x 5µ and ambient column temperature was maintained. The linearity of this method was found to be linear with a coefficient of regression at 0.999 in the concentration range of 50% to 160%. The linear regression equation was y=2846.0 x+89.55. The present developed HPLC method is detected to be suitable. The analytical solution was detected to be stable up to 48 Hrs at room temperature
