Gabor, F.

Neutsch, L.

Przylucka, A.

Yemelyanova, T. V.

Electronic archive of Tomsk Polytechnic University

240 XVI Международная научно-практическая конференция имени профессора Л.П. КулёваReferences 1. Vinogradov N.V., Vinogradov V.V., Tuzova O.L. Absorption metal surface min-eral lidit. The Way of Science // International scientific journal, 2014.– №4(4).– P.10–12. 2. Vinogradov N.V., Akunova D.A., Vinogradov V.V., Tuzova O.L. Preparation of Catalitic Surface of Lidit Powder // International Conference on European Science and Technology. Materials of the VII International Research and Practice Conference. Vol.II. 23–24 April, 2014.– Munich, Germany, 2014.– P.285–290.Expression of hydrophobins for protective coating of polymeric nanoparticles for drug deliveryT.V. Yemelyanova1,2, A. Przylucka1, L. Neutsch3, F. Gabor3Scientific supervisors – Associate Professor, I.S. Druzhinina1; DSc, Professor R.R. Ahmedzhanov41Research Division Biotechnology and Microbiology Institute of Chemical Engineering, Vienna University of Technology Austria, 1040, Vienna, 13 Karlsplatz, tatyana.yemelyanova@gmail.com2Institute of Natural Resources National Research Tomsk Polytechnic University Russia, 634050,Tomsk, 30 Lenin Avenue, tve5@tpu.ru3Pharmaceutical Technology and Biopharmaceutics Institute of Life Sciences, University of Vienna, Vienna, Austria4Institute of Non-Destructive Testing National Research Tomsk Polytechnic University Russia, 634050, Tomsk, 30 Lenin Avenue  The use of nanotechnology in medicine and more specifically in drug delivery extends rapidly. Nanoparticles, that are becoming important instru-ments in the area of medical treatment, have the potential to enhance the therapeutic efficacy of medicine. However, particle properties have to be carefully engineered in order to regulate drug release and рrolong the resi-dence time in the body. Currently, chemical stabilizers (e.g. poloxamers) with limited biocompatibility are used to facilitate preparation and ensure formu-lation stability. Alternative strategies for surface-modification are urgently searched after. A synergistic approach with multiple benefits would be to coat the nanocarriers with self-adhesive proteins. Hydrophobins are unique fungal amphiphilic proteins with a broad range of biосоmpаtibility with different materials. They are also considered to be suitable for surface modificationand biomolecule immobilization purposes also because they are immunolog-ically inert [1]. Thus, therapeutic agents such as enzymes can be encapsulat- Секция 7 Химия и химическая технология (на английском языке) 241ed by the nanocarriers coated with hydrophobins, thereby protecting these аgents against enzymatic dеgrаdаtion and rejection by the immune system. Purpose of this work was to compare the ability of Aspergillus	 nid-ulans DewA (class I) [2], Trichoderma	reesei HFB4 (class II) and HFB9b (non-class II, intergroup between class I and II) hydrоphobins labelled C- and N-terminally with the green fluorescent protein (GFP) to cover poly (lac-tic-co-glycolic acid) nanoparticles. For this, a production system in Escher-ichia	coli was set up for the recombinant expression of the respective fusion proteins. DewA-GFP was selected for the optimization of the expression us-ing parameters such as incubation temperature, cell density, inducer concen-tration and time of induction to increase the yield and avoid degradation of the target proteins. References 1. Mirkka Sarparanta et al. Journal of Molecular Pharmaceutics, 2012.– 9(3).– P.654–663. 2. Stephane Boeuf et al. Journal of Acta Biomaterialia. Volume 8, Issue 3, March 2012.– P.1037–1047.

Expression of hydrophobins for protective coating of polymeric nanoparticles for drug delivery

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