Eveillard, D.

Fleury, F.

Martinez, Sf

Nomme, J.

Renodon-Cornière, A.

Takahashi, M.

Weigel, P.

Biochimie

HAL Descartes

Targeting human Rad51 by specific DNA aptamers induces inhibition of homologous recombination

Susan F. Martinez

Axelle Renodon-Cornière

Julian Nomme

Damien Eveillard

Fabrice Fleury

Masayuki Takahashi

Pierre Weigel

Crossref

Hal-Diderot

International audienceHuman Rad51 (HsRad51), a key element of the homologous recombination repair pathway, is related to the resistance of cancer cells to chemo- and radio-therapies. This protein is thus a good target for the development of anti-cancer treatments. We have searched for new inhibitors directed against HsRad51 using the Systematic Evolution of Ligands by EXponential enrichment (SELEX) approach. We have selected three aptamers displaying strong effects on strand exchange activity. Analysis by circular dichroism shows that they are highly structured DNA molecules. Our results also show that they affect the first step of the strand exchange reaction by promoting the dissociation of DNA from the ATP/HsRad51/DNA complex. Moreover, these inhibitors bind only weakly to RecA, a prokaryotic ortholog of HsRad51. Both the specificity and the efficiency of their inhibition of recombinase activity offer an analytical tool based on molecular recognition and the prospect of developing new therapeutic agents

Martinez, Susan F

Renodon-Cornière, Axelle

Nomme, Julian

Eveillard, Damien

Fleury, Fabrice

Takahashi, Masayuki

Weigel, Pierre

HAL: Hyper Article en Ligne

Targeting human Rad51 by specific DNA aptamers induces inhibition of homologous recombination.

HAL-Univ-Nantes

https://hal.archives-ouvertes.fr/hal-00549787

Targeting human Rad51 by specific DNA aptamers induces inhibition of homologous recombination

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HAL Descartes

Crossref

Hal-Diderot

HAL: Hyper Article en Ligne

HAL-Univ-Nantes

HAL-Univ-Nantes