Drechsler, Nadine

Habekuß, Antje

Schubert, Jörg

Thieme, Thomas

English

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Nachwuchswissenschaftlerforum / Young Scientists Meeting 2011  Nadine Drechsler et al.  Berichte aus dem Julius Kühn-Institut 162, 2011  Variation in virus content among individual leaves of susceptible barley infected with Barley dwarf virus  Nadine Drechsler1, Antje Habekuß2, Thomas Thieme1, Jörg Schubert3 1BTL Bio-Test Labor GmbH Sagerheide 2Julius Kühn-Institut, Institute for Resistance Research and Stress Tolerance 3Julius Kühn-Institut, Institute for Biosafety of Genetically Modified Plants nadine.drechsler@jki.bund.de Wheat dwarf virus (WDV) and Barley dwarf virus (BDV) are circular single-stranded DNA-viruses belonging to the family Gemi-niviridae. Infected cereals are dwarfed, stunted, show yellow streaks and have a drastically reduced yield or die off early. WDV was first described in 1961 by VACKE in the Czech Republic. Since then it was found throughout Europe. In 2007 a strain infecting mostly barley was described as Barley dwarf virus (SCHUBERT et al.). It shows about 83-84% nucleotide identity with WDV. Both viruses are transmitted by the leafhopper Psammotettix alienus in a persistent non-propagative manner.   To study virus infection a very sensitive qPCR assay was developed. It allows detec-tion and quantification of both viruses due to primers in conserved regions of the coat protein gene. The highly susceptible winter barley ‘Rubina’ was inoculated with 3 viru-liferous leafhoppers under controlled greenhouse conditions. Samples of all leave tips were taken 7, 14, 21 and 28 days after inoculation (dpi) and analyzed with qPCR.  Viral copies were detectable in all leaf tips, but virus concentrations varied between leaves. The eldest leaf, which was the inoculated one, had a virus content in the range of 103 copies/30 ng plant DNA 7 dpi increasing to 105 copies at 28 dpi. The younger leaves had a higher virus DNA con-tent than the eldest one at every sampling date, the youngest reaching viral loads in the range of 108 copies 28 dpi. No signifi-cant difference could be found between the virus content of the tip and the base of the same leaf.   In conclusion it is advisable to harvest the youngest leaf tip to test plants for virus infection. For experiments where virus contents of plants are compared the leaf tip of the same level should be sampled.  SCHUBERT J, A. HABEKUß, K. KAZMAIER, H. JESKE (2007): Surveying cereal-infecting geminiviruses in Germany – diagnostics anddirect sequencing using rolling circle amplification. Virus Res. 127, 61–70. VACKE J. (1961): Wheat dwarf virus disease. Biol. Plant. (Praha) 3, 228-233.        61

Variation in virus content among individual leavesof susceptible barley infected with Barley dwarf virus

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Variation in virus content among individual leavesof susceptible barley infected with Barley dwarf virus

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