Beer, Martin

Hoffmann, Donata

Rubbenstroth, Dennis

Schlottau, Kore

Schulze, Vanessa*

Ulrich, Rainer

English

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Poster Presentations New and Re-Emerging Zoonotic Diseases 138 N08  Searching for the reservoirs of orthobornaviruses  Vanessa Schulze1, Kore Schlottau2, Donata Hoffmann2, Rainer G. Ulrich1, Martin Beer2 and Dennis Rubbenstroth2    1Friedrich-Loeffler-Institut, Insitute of Novel and Emerging Infectious Diseases, Greifswald-Insel Riems, Germany 2Friedrich-Loeffler-Institut, Institute of Diagnostic Virology, Greifswald-Insel Riems, Germany   Keywords: bornavirus, reservoir, encephalitis    Three mammalian orthobornaviruses are known so far: Borna disease viruses (BoDV) 1 and 2 (species: Mammalian 1 orthobornavirus) and the variegated squirrel bornavirus 1 (VSBV-1; Mammalian 2 orthobornavirus). The bicolored white-toothed shrew (Crocidura leucodon) is known as the natural reservoir of BoDV-1, whereas VSBV-1 was detected in different exotic squirrel species kept in captivity but its natural reservoir remains unknown. One objective of our investigations within the Zoonotic Bornavirus Consortium (ZooBoCo) is to identify the reservoirs of these viruses. For this purpose, we are following targeted and non-targeted sampling approaches. Small mammals, including rodents and insectivores, are tested by real-time reverse transcription-polymerase chain reactions (RT-qPCRs) targeting either BoDV-1/2, VSBV-1 or a broad spectrum of orthobornaviruses. A non-targeted investigation of approximately 5,000 small mammals from Germany did not show any positive samples. Targeted investigations of shrews in BoDV-1-endemic regions detected BoDV-1 in one of 20 bicolored white-toothed shrews. Sequence comparison confirmed a BoDV-1 sequence of the cluster 2 previously identified in Bavaria in encephalitis cases in horses and humans.  In conclusion, the wildlife reservoir of VSBV-1 still remains unclear, whereas our investigations on BoDV-1 confirmed the bicolored white-toothed shrew as reservoir of BoDV-1.   

Searching for the reservoirs of orthobornaviruses

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Searching for the reservoirs of orthobornaviruses

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