Trojnar, E.

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Posters 503Zoonoses 0503First comparison of genome sequences of rotavirus groups A to G reveals insights into evolution and demarcation of rotavirus species E. Trojnar1, E. Kindler2, P. Otto3, J. Matthijnssens4, G. Heckel5, *R. Johne1 1Federal Institute for Risk Assessment, Berlin, Germany 2Kantonal Hospital St. Gallen, Institute of Immunobiology, St. Gallen, Switzerland 3Friedrich Loeffl er Institute, Institute for Bacterial Infections and Zoonoses, Jena, Germany 4University of Leuven, Rega Institute for Medical Research, Leuven, Belgium 5University of Bern, Institute of Ecology and Evolution, Bern, Switzerland  Rotaviruses are a leading cause of acute viral gastroenteritis in humans and animals. Currently, fi ve rotavirus groups (A to E) have been defi ned based on antibody reactivity, and three additional putative groups (F, G and ADRV-N) are investigated. Group A, B, C and ADRV-N are found in humans and animals, whereas group D, F and G have only been detected in birds so far.Here, the fi rst nearly complete genome sequences of group F and G rotaviruses have been determined and were compared to known sequences of the other rotavirus groups. Generally, phylogenetic trees based on 10 of 11 genome segments revealed that all rotavirus groups were clearly separated. Nucleotide sequence identities varied between 33.4% and 64.5%. A threshold was defi ned for demarcation of rotavirus species based on the sequence of the VP6-encoding genome segment; according to this, ADRV-N was placed into a putative new group H. Phylogenetic analysis indicated two highly separated clades, one consisting of group A, C, D and F, and the other containing groups B, G, and H. Interestingly, the terminal sequences of the genome segments were also highly conserved within these clades. As - among other factors - these terminal sequences determine specifi city of genome segment packaging, these fi ndings may indicate the possibility of intergroup reassortments. A group A-like NSP1 gene was identifi ed in the group D rotavirus strain 05V0049, which may represent the fi rst example for an intergroup reassortant.    Corresponding author:Reimar JohneReimar.Johne@bfr.bund.de

First comparison of genome sequences of rotavirus groups A to G reveals insights into evolution and demarcation of rotavirus species

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First comparison of genome sequences of rotavirus groups A to G reveals insights into evolution and demarcation of rotavirus species

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