This study aimed to assess if GSM exposure (1.5W/kg-15min, 6W/kg-15 and 45min) was associated with further changes in cerebral function and biomarkers, in addition to glial fibrillary acidic protein (GFAP) increase previously demonstrated in the rat brain. Results showed a partial reproducibility on GFAP levels. Further biological responses were not dependent on SAR or exposure duration. These results strongly support the complexity of electromagnetic fields effect on biological responses and point out the need to assess the parameters of its variability