Leotia lubrica (Scop.) Pers. (Leotiaceae, Leotiales, Leotiomycetes, Ascomycota) was recorded for the first time from Argentina in Lago Puelo National Park, Chubut Province. Previously cited from the native forest in Chile, Los Lagos Region in southern South America, the species showed a distribution in temperate regions around the world. The description together with photographs are included.Leotia lubrica (Ascomycota, Leotiomycetes, Leotiales, Leotiaceae) se registra por primera vez para la Argentina en el Parque Nacional Lago Puelo, Chubut. La especie había sido previamente citada en los bosques nativos de Chile en la Región de los Lagos, en el sur de Sudamérica. La especie se distribuye en regiones templadas alrededor del mundo. Se incluye una descripción y fotografías de la especie.Fil: Lorenzo, Laura E.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Patagonia Norte. Instituto de Investigación en Biodiversidad y Medioambiente; ArgentinaFil: Messuti, Maria Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Patagonia Norte. Instituto de Investigación en Biodiversidad y Medioambiente; Argentin

Lorenzo, Laura E.

Messuti, Maria Ines

Darwiniana nueva serie

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Original recibido el 15 de abril de 2013, aceptado el 10 de setiembre de 2013Editor Asociado: Alicia López 237DARWINIANA, nueva serie 1(2): 237-240. 2013Versión final, efectivamente publicada el 30 de diciembre de 2013DOI: 10.14522/darwiniana.2013.12.523ISSN 0011-6793 impresa - ISSN 1850-1699 en líneaAbstract. Lorenzo, L. E. & M. I. Messuti. 2013. Leotia lubrica (Ascomycota, Leotiaceae) found in Patagonia, Ar-gentina. Darwiniana, nueva serie 1(2): 237-240.LEOTIA LUBRICA (ASCOMYCOTA, LEOTIACEAE) FOUND IN PATAGONIA, ARGENTINAInstituto de Investigaciones en Biodiversidad y Medio Ambiente (CONICET - Universidad Nacional del Comahue); Departamento de Botánica, Centro Regional Universitario Bariloche, Universidad Nacional del Comahue, Quintral 1250, 8400 San Carlos de Bariloche, Río Negro, Argentina. lauralor@gmail.com (author for correspondence).Laura E. Lorenzo & María Inés MessutiLeotia lubrica (Ascomycota, Leotiomycetes, Leotiales, Leotiaceae) was recorded for the first time from Argentina in Lago Puelo National Park, Chubut Province. It has been previously cited in the native forest of Chile, Los Lagos Region in southern South America. The species is distributed in temperate regions around the world. A description and photographs of the species are included.Keywords. Argentina; new record; Patagonian mycobiota, pileate Ascomycota.Resumen. Lorenzo, L. E. & M. I. Messuti. Leotia lubrica (Ascomycota, Leotiaceae) hallada en la Patagonia, Argen-tina. Darwiniana, nueva serie 1(2): 237-240.Leotia lubrica (Ascomycota, Leotiomycetes, Leotiales, Leotiaceae) se registra por primera vez para la Argentina en el Parque Nacional Lago Puelo, Chubut. La especie había sido previamente citada en los bosques nativos de Chile en la Región de los Lagos, en el sur de Sudamérica. La especie se distribuye en regiones templadas alrededor del mundo. Se incluye una descripción y fotografías de la especie.Palabras clave. Argentina; Ascomycota pileado; micobiota patagónica; nueva cita.INTRODUCTIONThe genus Leotia Pers. is placed in the family Leotiaceae (Korf & Lizoň, 2001) which includes discomycetes with pileate ascomata, with the ex-ternal layer of the ectal excipulum and a medullary layer immersed in abundant gel, and inoperculate asci. This genus encloses only few species: L. atra Weinm., L. chlorocephala Schwein. L. infundibu-liformis (Schaeff.) Fr., L. lubrica (Scop.) Pers., L. nana (With.) Fr. Most of them are widely distribut-ed especially in temperate regions (Akata & Kaya, 2010; Kirk et al., 2008). Particularly, the genus Le-otia has not been cited before for Argentina. Nev-ertheless, Pezoloma Clem., another member of the Leotiaceae, was cited scarcelly in our country from Neuquén, Río Negro, and Tierra del Fuego e Islas del Atlántico Sur Provinces (Gamundí et al., 2004). Within the framework of the study of diversity of the lichenized and non lichenized fungi in Pa-238DARWINIANA, nueva serie 1(2): 237-240. 2013tagonia, the species Leotia lubrica (Scop.) Pers., was recorded for the first time in Argentina in Lago Puelo National Park, Chubut Province. MATERIALS AND METHODSThe examined material was collected during the field trip, alongside Población Reitman trail in the Botanical Garden of Lago Puelo National Park, Chubut Province (42° 11´ S - 71º 42´ W). In this area, the native vegetation was replaced by oaks, maples, and fruit trees among others because the place was formerly occupied by a farm. Nowadays the Botanical Garden is characterized by a mixed flora with exotic and native species.Ascomata height, pileus broad, and stipe length were measured from dry specimens under dissect-Fig. 1. Leotia lubrica. A, habitat. B, inmature asci. C, ascus with uniseriate ascospores. D, paraphysis simple, capitate. E, paraphysis forked. F, dried ascomata (herbarium specimens). G, inmature guttulate ascospore. H, mature ascospore. From Lorenzo s.n. (BCRU 4979).ing microscope. Morphological and anatomical features of fungal specimens were observed and measured in water under a bright-field microscope (BF) and differential interference contrast micros-copy (DIC). Several reagents were used to observe specific microscopic structures: Melzer´s reagent, 5 % potassium hydroxide (KOH), and lactophenol cotton blue (LCB) (Gams et al., 1980). Micro-graphs were obtained with USB 2.0 digital cam-era. The specimens examined are kept in BCRU Herbarium (Thiers, 2013). The authors and synon-ymy of the species are recorded in Index Fungorum (www.indexfungorum.org).RESULTSLeotia lubrica (Scop.) Pers., Comm. fung. clav. (Lipsiae): 31. 1797. Helvella lubrica Scop., Fl. L. E. LORENZO & M. I. MESSUTI. Leotia lubrica from Argentina239Carniol., Edn 2 (Wien) 2: 477. 1772. TYPE: “Loc. typ. ignota, ad terram interfoliis in silvis, aestate-autumno”, date ?, col.? (?Herb. Persoon) (sensu Imai, 1941). Fig. 1.Ascomata gregarius to subcespitous, pileate estipitate, gelatinous, 38-50 mm; pileus convex, smooth, irregularly rounded or lobed, inrolled mar-gin, viscid when moist, yellowish to yellow-green, olivaceous buff when dry, 7 mm diam., KOH + yel-lowish green in section; stipe central, cylindrical, concolorous with the pileus, yellowish when dry, covered distally with greenish granules, hollow when mature. Asci 8-spored, inoperculate, cylin-drical, I-, 130-157 x 7-10 µm. Paraphyses simple to forked, septate, capitate, 2.4 µm diam. Ascospo-res 1-2 seriate, hyaline, asymmetrical, subfusiform with rounded ends, slightly curved, guttulate, 17-25 x 5-6.5 µm.Iconography. Akata & Kaya, 2010: 2; Furci Geor-ge-Nascimento, 2007: 61; Gamundí, 1979: 92. Distribution and habitat. Leotia lubrica was previously recorded in southern South Ameri -ca from the native forest in Chile, in Los Lagos Region (Gamundí, 1979; Gamundí et al., 2004; Furci George-Nascimento, 2007). Leotia lubrica has been found in the phytogeographical area of Deciduous-Valdivian forest, Subantartic Province (Cabrera, 1994) in Parque Nacional Lago Puelo, Chubut Province; growing gregariously on soil beu-tween plant debris. This record is the first report of the genus Leotia in Argentina. Fig. 2.Observations. The species of Leotia have tradi-tionally been defined by the fresh color of the as-comata. However, a molecular phylogenetic study revealed that color is a poor predictor of species affiliation, suggesting that further study is need-ed to find new and more predictive species lim-its (Zhong & Pfister, 2004). The morphology and anatomy of the material collected in Parque Nacio-nal Lago Puelo agree with the descripton given by Gamundi (1979) of Leotia lubrica recorded in Os-orno, Chile, in Nothofagus obliqua (Mirb.) Oerst. (“roble pellín”) forest. Some authors (e.g. Leimona & Velasco, 1975; Gamundí, 1979) consider that the species has 1-cellular and guttulate ascospores. Nevertheless we agree with other authors (e.g. Dennis, 1981; Akata & Kaya, 2010) who showed that the ascospores are multisepate, although the septa are difficult to observe due to the presence of guttules with the classic techniques of microscopy (Fig. 1H).Persoon´s collections are deposited in L Her-barium (Netherlands): five of them correspond to L. lubrica: L 0116427, L 0116428, L 0116430, L 0116429, L 0116431. None of these is the type specimen (Thijsse, Senior Collection Manager, pers. com.). More synonyms can be consulted in Index Fungorum (http://www.indexfungorum.org/) and Imai (1941).Examined materialARGENTINA. Chubut. Depto. Cushamen, Parque Nacional Lago Puelo, Jardín Botánico, on Fig. 2. Distribution of Leotia lubrica in Patagonia, sou-thern South America. 1, Chile, Los Lagos Region. 2, Ar-gentina, Chubut Province, Parque Nacional Lago Puelo.240DARWINIANA, nueva serie 1(2): 237-240. 2013soil among fallen leaves of Acer pseudoplatanus (maple), 3-V-2008, Lorenzo s.n. (BCRU 4979).AKNOWLEDGEMENTSFunds for this research were provided by Univer-sidad Nacional del Comahue and Consejo Nacional de Investigaciones Científicas y Técnicas. Further thanks to Administración de Parques Nacionales (Argentina), Guardaparque Marcos Ocampo of Lago Puelo National Park, and to Esteban Báez.BIBLIOGRAPHYAkata, I. & A. Kaya. 2010. A new jelly ascomycetous genus re-cord for Turkish Mycobiota. SDU Journal of Science 5: 1-4.Cabrera, A. L. 1994. Regiones fitogeográficas argentinas, 1a reimpresión, Enciclopedia Argentina de Agricultura y Jar-dinería: tomo 2, pp. 1-85. Buenos Aires: ACME. S.A.C.I.Dennis, R. W. G. 1981. British Ascomycetes. Vaduz: J. Cramer.Furci George-Nascimento, G. M. 2007. Fungi Austral. Guía de campo de los hongos más vistosos de Chile. Concepción: Corporación Chilena de la Madera.Gams, W. H. A. van der Aa, A. J. van der Plaats-Niterink, R. A. Samson & J. A. Stalpers. 1980. CBS Course of Mycology. 2nd ed. Baarn: Centraalbureau voor Schimmelcultures.Gamundí, I. J. 1979. Subantartic Geoglossaceae II. Sydowia 32: 86-98.Gamundí, I. J.; D. W. Minter, A. I. Romero, V. A. Barrera, A. L. Giaiotti, M. I. Messuti & M. Stecconi. 2004. Checklist of the Discomycetes (Fungi) of Patagonia,  Tierra del Fuego and adjacent Antarctic areas. Darwiniana 42: 63-164.Imai, S. 1941. Geoglossaceae Japoniae. Journal of Faculty of Agriculture, Hokaido Imperial University 45: 155-264.Kirk, P. M.; P. F. Cannon, D. W. Minter & J. A. Stalpers (eds.). 2008. Dictionary of the Fungi. 10th. ed. Wallingford: CAB International.Korf, R. P. & P. Lizoň. 2001. The status of the ordinal name Leotiales. Czech Mycology 52: 255-257.Leimona, X. & E. Velasco. 1975. Sobre algunas geoglosáceas (Helotiales, Ascomycetes) observadas en Catalunia. Anales del Instituto Botánico A. J. Cavanilles 32: 101-110.Thiers, B. [continuously updated, accessed 2013] Index herbar-iorum: a global directory of public herbaria and associat-ed staff. New York Botanical Garden’s Virtual Herbarium, http://sweetgum.nybg.org/ihZhong, Z. & D. H. Pfister. 2004. Phylogenetic relationships among species of Leotia (Leotiales) based on ITS and RPB2 sequences. Mycological Progress 3: 237-246. DOI: http://dx.doi.org/10.1007/s11557-006-0094-8.

Leotia lubrica (Ascomycota, Leotiaceae) hallada en la Patagonia, Argentina

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Leotia lubrica (Ascomycota, Leotiaceae) hallada en la Patagonia, Argentina

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