Arsanto, J.P.

Birnbaum, D.

Borg, J.P.

Hatzfeld, M.

Jaulin-Bastard, F.

Le Bivic, A.

Marchetto, S.

Navarro, C.

Saito, H.

Santoni, M.J.

Vely, F.

Integrity of epithelial tissues relies on the proper apical-basolateral polarity of epithelial cells. Members of the LAP (LRR and PDZ) protein family such as LET-413 and Scribble are involved in maintaining epithelial cell polarity in Caenorhabditis elegans and Drosophila melanogaster, respectively. We previously described Erbin as a mammalian LET-413 homologue interacting with ERBB2/HER2, an epidermal growth factor receptor family member. Erbin and ERBB2/HER2 are located in the basolateral membranes of epithelial cells. We show here that Erbin interacts with p0071 (also called plakophilin-4), an armadillo repeat protein linked to the cytoskeleton. Erbin binds to p0071 in vitro and in vivo in a PDZ domain-dependent manner, and both proteins colocalized in desmosomes of epithelial cells. Using a dominant negative approach, we found that integrity of epithelial cell monolayer is impaired when interaction between Erbin and p0071 is disrupted. We propose that Erbin is connected by p0071 to cytoskeletal networks in an interaction crucial for epithelial homeostasis

Jaulin-Bastard, Fanny

Arsanto, Jean-Pierre

Le Bivic, André

Navarro, Christel

Vély, Frédéric

Saito, Hiroko

Marchetto, Sylvie

Hatzfeld, Mechthild

Santoni, Marie-Josée

Birnbaum, Daniel

Borg, Jean-Paul

HAL AMU

Interaction between Erbin and a Catenin-related protein in epithelial cells

https://hal.archives-ouvertes.fr/hal-00306521

Interaction between Erbin and a Catenin-related protein in epithelial cells

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